2010-09-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/684512摘要：間葉幹細胞可分離自骨膜、骨小樑、脂肪組織、關節液、骨骼肌及脫落之牙齒，其在特定培養環境下具高增殖能力及可分化為多種細胞系特性，由許多臨床前及臨床之研究試驗中已證實間葉幹細胞在再生醫學及組織工程具應用之潛力及治療價值。此外，攜報導基因的間葉幹細胞經移植後之表現穩定性將有助於確認其在體內之功能及分化潛能。分離自表現報導基因之骨髓幹細胞具有相同之表現型式，此策略有助於追蹤其移植及分化後之命運。因此本研究將 育成表現螢光基因之第一代公豬五轉殖系，各系製作冷凍精液30劑，合計150劑供種原保存於液氮桶中備用。 螢光豬完整體細胞之種原保存共二十株細胞。建立二十株源自螢光豬骨髓之間葉幹細胞，經寄存於財團法人食品工業發展研究所生物資源保存中心，並於本年度提出專利申請。<br> Abstract: Mesenchymal stem cells (MSCs) have been isolated from bone marrow, periosteum, trabecular bone, adipose tissue, synovium, skeletal muscle and deciduous teeth. They also can be expanded with high efficiency, and induced differentiation into multiple lineages under defined culture conditions. These cells have generated a great deal of interest because of their potential use in regenerative medicine and tissue engineering, and there are some dramatic examples, derived from both pre-clinical and clinical studies, that illustrate their therapeutic value. In addition, consistent expression of reporter gene incorporating into genome of implanted MSCs will facilitate the progress for verifying their functions and differentiation potential in vivo. The bone marrow-derived MSCs isolated from transgenic animal expressing reporter gene possessed the characteristics of identical expression pattern, and this strategy is f a v o r a b l e t o t r a c k t h e i r f a t e a f t e r t r a n s p l a n t a t i o n a n d differentiation.Hence, The fist year plan to breed five transgenic line from fluorescent pigs expressing different reporter gene (green fluorescence protein (EGFP) and enhanced red fluorescence protein (ERFP) and make freezing semen 30 doses each, totally 150 doses for germplsma preservation. In addition, prepare 20 lines skin fibroblast and marrowderived mescenchymal stem cells ready to keep in Bioresource Collection and Research Center, Food Industry Research and Development Institute for patent apply in this year.Green fluorescent proteinMesenchymal stem cellsGreen pigs3. 骨髓間葉幹細胞螢光豬螢光蛋白質