Chen I.-T.Chang L.-K.Lin S.-S.Kou G.-H.Chu C.-Y.Lo C.-F.C. Y. ChuCHIA-YING CHUSHIH-SHUN LINL. K. Chang2020-02-102020-02-10201716643224https://scholars.lib.ntu.edu.tw/handle/123456789/457014Members of the microRNA miR-10 family are highly conserved and play many important roles in diverse biological mechanisms, including immune-related responses and cancerrelated processes in certain types of cancer. In this study, we found the most highly upregulated shrimp microRNA from Penaeus vannamei during white spot syndrome virus (WSSV) infection was miR-10a. After confirming the expression level of miR-10a by northern blot and quantitative RT-PCR, an in vivo experiment showed that the viral copy number was decreased in miR-10a-inhibited shrimp. We found that miR-10a targeted the 5' untranslated region (UTR) of at least three viral genes (vp26, vp28, and wssv102), and plasmids that were controlled by the 5' UTR of these genes produced enhanced luciferase signals in transfected SF9 cells. These results suggest a previously unreported role for shrimp miR-10a and even a new type of host-virus interaction, whereby a co-opts the key cellular regulator miR-10a to globally enhance the translation of viral proteins. ? 2017 Huang, Kang, Chen, Chang, Lin, Kou, Chu and Lo.[SDGs]SDG3microRNA; Article; centrifugation; dual luciferase assay; gene expression; gene sequence; medical procedures; nonhuman; Northern blotting; Penaeus; polymerase chain reaction; real time polymerase chain reaction; reverse transcription polymerase chain reaction; RNA extraction; Sf9 cell line; shrimp; virus cell interaction; virus gene; virus infection; virus replication; vp26 gene; vp28 gene; Western blotting; white spot syndrome infection; White spot syndrome virus; wssv102 genejournal article10.3389/fimmu.2017.010842-s2.0-85028990103https://www2.scopus.com/inward/record.uri?eid=2-s2.0-85028990103&doi=10.3389%2ffimmu.2017.01084&partnerID=40&md5=e1d4a360bd41da19dbfcf0b12c0c8708