2010-08-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/643039摘要：本計畫擬以原子力顯微術作為檢測平台探討活性奈米化合物與流感病毒之交互作用機制，應用於流感病毒防疫及預防醫學。原子力顯微術是從事奈米科技研究中不可或缺的重要檢測儀器及製作工具，可解決觀測奈米材料及奈米元件尺寸極小的艱鉅問題，本試驗將合成能抑制流感病毒感染之活性奈米化合物，利用其崩解流感病毒顆粒方法與新思維，未來擬將已開發合成之抗流感病毒奈米分子應用於生醫防護資材上。本研究以創新思考及嶄新研究工具深入奈米量測之顯微分子領域，以分子作用直接證據來探討活性奈米化合物對流感病毒的作用機制，除透過原子力顯微術在特定生理條件下研究表面型態之變化外，並針對衍生之導電型原子力顯微術進行電流分佈與任意點之I/V 特性之研究。本計劃未來三年將就流感病毒表面力學與電學進行一系列之研究，希望就流感病毒表面行為作一個深入且完整的分析。本計畫執行成果有助於將活性奈米化合物應用於流感或禽流感相關防疫材料之生產，不僅加強前線防疫工作人員的保護裝備，相關產品易有莫大的國際流通潛能，大大提高跨領域應用的成效。<br> Abstract: The potentialities of the atomic force microscopy (AFM) make it a tool of undeniablevalue for the study of biologically relevant samples. AFM is a critical for developingnew biotechnological and biomedical applications and evenly crossing the limits ofthe traditional areas of application. A variety of biological samples can be imaged bythe AFM under environments that range from vacuum to ambient to liquid. AFM isnondestructive and nonperturbing, thus can be applied to soft biological samples.AFM topographic imaging has many biophysical applications, including visualizationof chemical properties on surfaces of biological samples under physiologicalconditions, subtle modifications during cell growth or treatment with drugs, andlocalization of specific active site on cells and virus. In the investigations of viruses,AFM is sufficiently incisive and reproducible because even the individual capsomerescan be visualized on the surfaces of both plant and animal viruses. And we could getthe conductivity (electric current distribution) and the modulation of current-voltage(I-V) behavior by conductive-atomic force microscopy (c-AFM). The study of thisproject will focus on nano-scaled mechanical and electrical properties of viral surfacewithin three years.原子力顯微術導電式原子力顯微術流感病毒AFMc-AFMinfluenza virus