Hsieh, Han YunHan YunHsiehLee, Che YiChe YiLeeChao, Jian ChieJian ChieChaoFan, Yu JuiYu JuiFanHORN-JIUNN SHEEN2023-11-242023-11-242022-01-019781733419048https://scholars.lib.ntu.edu.tw/handle/123456789/637356First, we use microelectromechanical system (MEMS) technology to fabricate devices with different geometric structures on indium tin oxide (ITO) glass. The devices will be connected to an external electrical signal source to make the cells captured into the etched holes with negative dielectrophoresis (nDEP) to achieve cell manipulation. Second, we modulate the frequency of the electrical signal to achieve cell electroporation, and stain the cells with CFDA-SE and calcein to verify that after electropration, substances can be delivered from inside or outside the cell, and to find the optimal device for shRNA delivery.Dielectrophoresis | Electroporation | ITO glass | MEMS | shRNA deliveryDEVELOPMENT OF MICROWELL ARRAY FOR CELL TRAPPING AND SHRNA DELIVERY USING ELECTROPORATIONconference paper2-s2.0-85175717994https://api.elsevier.com/content/abstract/scopus_id/85175717994