李美慧2006-07-252018-06-282006-07-252018-06-282000http://ntur.lib.ntu.edu.tw//handle/246246/11375最近兩三年環境荷爾蒙對人及野生動 物之影響受到各界重視，因此發展標準的 測試環境荷爾蒙的方法乃為世界各先進國 家的當務之急。本研究希望能發展出整合 型的母幼鼠急性反應測試方法，不僅只是 可檢測環境污染物所具有之雌性荷爾蒙特 性，還可檢測出對肝臟酵素系統、甲狀腺 素與神經系統之影響。第一年本研究是利 用此生物測試系統檢驗多氯聯苯PCB 132 (2,2’,3,3’,4,6’- hexachlorobiphenyl) 及PCB 149 (2,2’,3,4’5’6-hexachlorobiphenyl)是否 有之雌性荷爾蒙特性及對肝臟酵素系統之 影響。從研究結果發現PCB132及PCB149 在測試的三個劑量下，都沒有造成母幼鼠 的子宮重增加的情形。PCB132及PCB149 對母幼鼠的肝臟P4501A酵素系統沒有影 響。另外，PCB 149有增加母幼鼠的肝臟 酵素P450 2B(PROD及BROD)活性的趨 勢，在對母幼鼠的肝臟P450酵素系統影響 是較類似PB-type inducer，與本研究原先的 假說相符，即在聯苯結構的2,3,6位置含氯 原子之多氯聯苯異構物，具有”不與戴奧辛 類似”之毒性。Recently, there is increasing public and scientific concern that some environmental pollutants may cause hormone disruption in human and wildlife. Therefore, there is an urgent need to develop an effective assay which can detect environmental hormone. In this study, an integrated bioassay which using acute responses of prepubertal female rats will be expanded and developed to examine effects of PCB 132 (2,2’,3,3’,4,6’- hexachlorobiphenyl) and PCB 149 (2,2’,3,4’5’6-hexachlorobiphenyl) on estrogenicity, hepatic enzyme activities, thyroxine, and brain dopamine as well as norepinephrine levels. First year of this project, it was focused to standardize the methods of the uterotropic responses as well as hepatic enzyme activities. The result of this study showed that PCB 132 and PCB 149 did not produce any uterotropic responses in prepubertal female rats at 8 mg/kg, 32 mg/kg and 96 mg/kg. PCB 132 and PCB 149 did not increase liver microsomal P450 1A enzyme activities of prepubertal female rats. However, there was an increase P450 2B activity at prepubertal female rats administered PCB 149 at 96 mg/kg. It indicated that PCB 149 was a PBtype enzyme inducer. The toxic effects of 2,3,6-substitiued PCB congeners were nondioxin- like.application/pdf36959 bytesapplication/pdfzh-TW國立臺灣大學地理環境資源學系暨研究所多氯聯苯雌性荷爾蒙肝臟酵素母幼鼠急性反應測試方法Polychlorinated biphenylEstrogenic effectsHepatic enzymesPrepubertal female ratsAcute Response bioassay[SDGs]SDG11reporthttp://ntur.lib.ntu.edu.tw/bitstream/246246/11375/1/892320B002024.pdf