Tung, Chih-kuanChih-kuanTungPeriasamy, AmmasiAmmasiPeriasamySo, Peter T. C.Peter T. C.SoChiu, Tsung-KaiTsung-KaiChiuLo, WenWenLoWang, Po-HsiangPo-HsiangWangSUNG-JAN LINJee, Shiou-HwaShiou-HwaJeeDong, Chen-YuanChen-YuanDong2009-02-182018-06-292009-02-182018-06-2920030277786Xhttp://ntur.lib.ntu.edu.tw//handle/246246/132756https://www.scopus.com/inward/record.uri?eid=2-s2.0-0141605430&doi=10.1117%2f12.478026&partnerID=40&md5=1b0b60371a1342689ac43b2da4bccf1aIndex mismatch induced spherical aberration is studied by comparing imaging ability of different immersion objectives in both uniformly fluorescent solutions and fluorescently labeled skin samples using scanning two-photon fluorescence microscopy. We investigated the performances of the objectives (air, water, glycerin, and oil immersion) by measuring the fluorescence profiles at different depths. In homogeneous fluorescent samples, we found that immersion medium with compatible refractive index as the samples yields better results, and small differences in refractive indices did not cause noticeable effects. Similar results were found in skin samples. Except for the air objective, we found that the choice of immersion medium did not have significant effects for in-depth imaging in the fluorescent solutions or skin samples.application/pdf693796 bytesapplication/pdfen-USFluorescence; Imaging; Microscopy; Skin; Spherical aberration; Two-photonAberrations; Biomedical engineering; Fluorescence; Microscopic examination; Multiphoton processes; Refractive index; Tissue; Two-photon fluorescence microscopy (TPFM); Medical imaging10.1117/12.4780262-s2.0-0141605430http://ntur.lib.ntu.edu.tw/bitstream/246246/132756/1/09.pdf