Hung, Hao-ChienHao-ChienHungTSUI-LIEN MAOFan, Ming-HueiMing-HueiFanHuang, Guan-ZhiGuan-ZhiHuangMinhalina, Ainani PrizaAinani PrizaMinhalinaChen, Chi-LongChi-LongChenLiu, Chao-LienChao-LienLiu2024-03-292024-03-2920241837-9664https://scholars.lib.ntu.edu.tw/handle/123456789/641619The three-dimensional (3D) cell culture technique has been applied comprehensively as a variable platform for medical research, biochemical signal pathway analysis, and evaluation of anti-tumor treatment response due to an excellent recapitulation of a tumor microenvironment (TME) in the in vitro cultured cancer cells. Pancreatic cancer (PaC) is one of the toughest malignancies with a complex TME and refractory treatment response. To comprehensively study the TME of PaC, there is an eager need to develop a 3D culture model to decompose the cellular components and their cross interactions. Herein, we establish a 3D PaC culture system with cancer stem cell (CSC) and scalability properties. To validate our model, we tested the individual PaC cell and the combined effects with cancer-associated fibroblasts (CAFs) on cancer tumorigenicity, the cellular interaction through the CXCR3/CXCL10 axis, and cellular responses reflection of anti-cancer treatments. With the help of our 3D technology, a simulated malignant spheroid with important stromal populations and TME physiochemical properties may be successfully recreated. It can be used in a wide range of preclinical research and helpful in advancing basic and translational cancer biology.enCXCR3/CXCL10 axis; Pancreatic cancer (PaC); cancer-associated fibroblast (CAF); spheroid model; therapeutic discovery.; three dimensional (3D); two dimensional (2D)[SDGs]SDG3journal article10.7150/jca.87494384955002-s2.0-85187694351https://api.elsevier.com/content/abstract/scopus_id/85187694351