Cheng, Yu JungYu JungChengWu, Ying JouYing JouWuLee, Fang WeiFang WeiLeeOu, Ling YiLing YiOuChen, Chi NanChi NanChenChu, Yu YingYu YingChuYEN-CHOU KUAN2022-12-082022-12-082022-082223-7747https://scholars.lib.ntu.edu.tw/handle/123456789/625906Anthracnose caused by Colletotrichum leads to a tremendous post-harvest mango loss. While chemical fungicides are applied to control anthracnose, natural alternatives are preferred due to food safety and environmental concerns. Pomelo extract (PE) exhibits a broad spectrum of antimicrobial activities; however, its effect against anthracnose is unknown. Here we investigated the chemical profile of PE using GC-MS and the anti-anthracnose activity of PE using in vitro and in vivo assays. We also evaluated the impact of storage temperature (0°, 5°, 10°, 20°, −20°, and −80 °C) and light conditions on the composition and antifungal activity of PE. We found that PE inhibited C. gloeosporioides in vitro with an IC50 of 3.2 mL L−1. Applying chitosan-based coating incorporated with 20 mL L−1 PE significantly suppressed anthracnose in post-harvest ‘Keitt’ mango. A storage temperature below 5 °C substantially preserved major compounds and the antifungal activity of PE after 6 m of storage. Finally, we showed that applying d-limonene, the key constituent of PE, inhibited C. gloeosporioides in vitro (IC50: 10.9 mM) and suppressed anthracnose in vivo. In conclusion, we demonstrated that the application of PE and d-limonene are sustainable methods for anthracnose control in post-harvest crops and established the preservation protocol for PE.enanthracnose | antifungal | edible coating | mango | pomelo peel | post-harvest disease[SDGs]SDG3journal article10.3390/plants11152064359565422-s2.0-85136960628WOS:000838999000001https://api.elsevier.com/content/abstract/scopus_id/85136960628