周祖述2006-07-262018-07-062006-07-262018-07-062001http://ntur.lib.ntu.edu.tw//handle/246246/27482Regulatory expression systems, such as tetracycline repressible system, ecdysone inducible system, and Lac operon inducible system, have been proved of tremendous value for researchers wishing to understand the functional significance of their genes of interest. In the post-genomic era, tight and inducible expression system is of extreme importance in the identification of molecular targets genuine for disease pathogenesis and drug development. In this proposal, a simple and efficient strategy is outlined to replace the traditional individual clone-based screening of generating regulatory expression system. Taking advantage of a GFP reporter construct under ecdysone responsive element and FACSorting based upon a simplistic positive and negative selection scheme, we had been able to isolate population of HEK293 and MDCK cells tightly regulated by ecdysone in term of the expression activity of exogenously introduced gene. Our preliminary data also showed similar strategy could be exploited to set up tetracycline inducible system. An example is provided to demonstrate how a dual regulatory expression system, which conjugates ecdysone inducible and tetracycline regulated systems, could be used to study the interplay of two closely related signaling molecules.application/pdf452065 bytesapplication/pdfzh-TW國立臺灣大學醫學院臨床醫學研究所Ecdysone inducible system, Tetracycline regulatory expression system, and LacSwitch expression system, Regulatory expression systemjournal articlehttp://ntur.lib.ntu.edu.tw/bitstream/246246/27482/1/892323B002010.pdf