Truong, Chi-MinhChi-MinhTruongJair, Yung-ChengYung-ChengJairChen, Hong-PoHong-PoChenChen, Wei-ChihWei-ChihChenLiu, Yi-HsinYi-HsinLiuChen, Pin-ChuanPin-ChuanChenPAI-SHAN CHEN2024-03-272024-03-272024-04-1500032670https://scholars.lib.ntu.edu.tw/handle/123456789/641449High-resolution matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) and nuclear magnetic resonance (NMR) spectroscopy are powerful tools to identify unknown psychoactive substances. However, in complex matrices, trace levels of unknown substances usually require additional fractionation and concentration. Specialized liquid chromatography systems are necessary for both techniques. The small flow rate of nano LC, typically paired with MALDI-TOF MS, often results in prolonged fractionation times. Conversely, the larger flow rate of semi-preparative LC, used for NMR analysis, can be time-consuming and labor-intensive when concentrating samples. To address these issues, we developed an integrated automatic system that integrated to regular LC.enAutomation; Fractioning and spotting; Liquid chromatography; Matrix-assisted laser desorption/ionization-time of flight-mass spectrometry; Nuclear magnetic resonance spectroscopyjournal article10.1016/j.aca.2024.342401384623402-s2.0-85186533162https://api.elsevier.com/content/abstract/scopus_id/85186533162