Wu, Chih-YuChih-YuWuLo, Chu-FangChu-FangLoHuang, Chang-JenChang-JenHuangYu, Hon-TsenHon-TsenYuWang, Chung-HsiungChung-HsiungWang2009-06-242018-07-062009-06-242018-07-06200200426822http://ntur.lib.ntu.edu.tw//handle/246246/161598https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036060435&doi=10.1006%2fviro.2001.1344&partnerID=40&md5=f25475c4cd8d25d1b91451c6cd3110fePerina nuda picorna-like virus (PnPV) is an insect-infecting RNA virus with morphological and physicochemical characters similar to the Picornaviridae. In this article, we determine the complete genome sequence and analyze the gene organization of PnPV. The genome of PnPV consists of 9476 nucleotides (nts) excluding the poly(A) tail and contains a single large open reading frame (ORF) of 8958 nts (2986 codons) flanked by 473 and 45 nt noncoding regions on the 53 and 33 ends, respectively. Northern blotting did not detect the presence of any subgenomic RNA. The PnPV genome codes for four structural proteins (CP1-4), and determination of their N-terminal sequences by Edman degradation, showed that all four are located in the 5′ region of the genome. The 3′ part of the PnPV genome contains the consensus sequence motifs for picornavirus RNA helicase, cysteine protease, and RNA-dependent RNA polymerase (RdRp) in that order from the 5′ to the 3′ end. In all of these characters, the genome organization of PnPV resembles the mammalian picornaviruses and two other insect picorna-like viruses, infectious flacherie virus (IFV) of the silkworm and Sacbrood virus (SBV) of the honeybee. In a phylogenetic tree based on the eight conserved domains in the RdRp sequence, PnPV formed a separate cluster with IFV and SBV, which suggests that these three insect picorna-like viruses might constitute a novel group of insect-infecting RNA viruses. © 2002 Elsevier Science (USA).application/pdf482647 bytesapplication/pdfen-USComplete genome sequence; Insect; Perina nuda; Picornavirus; PnPVcysteine proteinase; genomic RNA; nucleotide; RNA helicase; RNA polymerase; structural protein; virus enzyme; amino terminal sequence; article; codon; consensus sequence; DNA flanking region; flachie virus; gene sequence; genetic code; genome analysis; honeybee; insect; mammal; nonhuman; Northern blotting; nucleotide sequence; open reading frame; Perina nuda Picorna like virus; phylogenetic tree; Picornavirus; priority journal; protein analysis; protein degradation; RNA virus; sacbrood virus; silkworm; viral genetics; virus genome; virus infection; Apis mellifera; Bombyx mori; Infectious flacherie virus; Insecta; Mammalia; Nuda; Perina nuda picorna-like virus; Picornaviridae; RNA viruses; Sacbrood virusjournal article10.1006/viro.2001.1344120098732-s2.0-0036060435http://ntur.lib.ntu.edu.tw/bitstream/246246/161598/1/14.pdf