2012-08-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/648540摘要：全氟碳化合物(perfluorinated chemicals, PFCs)為具環境持久性和生物累積性的新興有機汙染物，在人體內甚至臍帶血中都能檢測到該類物質。動物實驗已報導PFCs能誘發免疫毒性效應，其可能的作用途徑為PFCs活化過氧化物酶體增殖物激活受體(peroxisomeproliferator-activated receptor, PPAR)，進而影響細胞激素(cytokines)的表現；另也被證實可能會刺激免疫球蛋白E對外來過敏原的免疫反應。然而依目前有限研究資料仍不足以回答全氟碳化合物暴露是否影響人類免疫系統進而引起兒童過敏疾病。本計畫預計利用三年時間進行兩個出生世代追蹤比較研究，除了持續追蹤台灣出生世代(Taiwan BirthPanel Study, TBPS I)八至十歲孩童，探討其PFCs暴露濃度與不同過敏疾病發展之相關性外；更將擴展研究對象至全國性出生世代(TBPS II)，以更具全國代表性的樣本來探討PFCs暴露在兒童過敏性疾病所扮演的角色。本研究將以極致液相層析/串聯式質譜儀(UPLC/MS/MS)分析血清中全氟碳化合物濃度，並利用標準化過敏問卷、肺功能檢測、量測血清中免疫球蛋白E、特異過敏原免疫球蛋白E和細胞激素，以及人類周邊單核細胞(PBMC)刺激試驗，進而探究與體內全氟碳化合物之相關性以及其可能的致病機轉。此外，本研究亦將更進一步探討全氟碳化合物與鄰苯二甲酸酯類共暴露對兒童過敏疾病之影響。<br> Abstract: Perfluorinated compounds (PFCs) is recognized as one of the persistent andbio-accumulative emerging organic contaminations, and can even be detected inhuman umbilical cord blood. In animal studies, PFCs have been shown to induceimmunotoxicity through activation of the peroxisome proliferator- activated receptor(PPAR) pathway and then leading to further changes of cytokines. In addition, PFCswill also enhance allergic reaction via increasing IgE production to allergens.However, few studies have investigated the health impact of PFCs on human immunesystem and subsequent allergic diseases in children. This three-year project mainlyfocus on two parallel birth cohorts, the Taiwan Birth Panel Study I (TBPS I) withchildren aged from 8 to 10 and the Nationwide Taiwan Birth Panel Study II (TBPS II)with children less 2 years old, to evaluate the association between PFCs exposure andoccurrence of different allergic diseases for different generations. We will measureserum PFCs concentration by Ultra-Performance Liquid Chromatography/ TandemMass Spectrometry (UPLC/MS/MS); use the International Study of Asthma andAllergies in Childhood (ISSAC) standardized questionnaires, pulmonary function test,total and allergen-specific serum immunoglobulin E, measurement of cytokines, andperipheral blood mononuclear cells (PBMC) stimulation test, to explore theassociation and possible mechanism. Moreover, multiple chemical exposures are ofincreasing concern, and we will also assess the co-exposure effect between PFCs andphthalate on children’s allergic diseases.過氧化物酶體增殖物激活受體細胞激素鄰苯二甲酸酯類台灣出生世代極致液相層析串聯式質譜儀人類周邊單核細胞PPARcytokinephthalateTaiwan Birth Panel StudyUPLC/MS/MSPBMC