Yang S.-J.CHIEN-CHING HUNGSUI-YUAN CHANGLee K.-L.Chen M.-Y.2021-12-012021-12-0120111386-6532https://www.scopus.com/inward/record.uri?eid=2-s2.0-79953741941&doi=10.1016%2fj.jcv.2011.01.017&partnerID=40&md5=2c5cc7dac7cc6c2a7980ff960cd33d98https://scholars.lib.ntu.edu.tw/handle/123456789/588875Background: The transmission routes of PARV4 are not completely understood. The first PARV4 serological study suggested that PARV4 is transmitted predominantly through parenteral route. Objectives: To set up an immunoblot system for studying the epidemiology of PARV4 infection in HIV-1 infected patients in Taiwan. Study design: Recombinant fusion proteins SUMOVP2 (a.a. 272-630 of PARV4 open reading frame 2) and SUMOVP3 (a.a. 604-914) were made and used as antigens in immunoblot. Plasma samples were from HIV-1 infected intravenous drug users IDU (69), homosexuals (66) and heterosexuals (68). Results: PARV4 IgG seropositive rate was 73.9%, 71.2% and 58.8%; IgM seropositive rate was 40.5%, 16.7% and 17.6% in IDUs, homosexuals and heterosexuals, respectively. Longitudinal samples were available from two homosexuals positive for IgM anti-PARV4, persistent IgM response was found over a period of 9 and 21 months, respectively. Conclusions: PARV4 is a common viral infection in HIV-1 infected homosexuals and heterosexuals in Taiwan. The detection of IgM anti-PARV4 does not always suggest recent PARV4 infection. ? 2011 Elsevier B.V.[SDGs]SDG3hybrid protein; immunoglobulin G; immunoglobulin M; protein sumovp2; protein sumovp3; unclassified drug; article; blood analysis; heterosexuality; homosexuality; human; Human immunodeficiency virus 1; Human immunodeficiency virus 1 infection; Human parvovirus 4; immunoblotting; major clinical study; nucleotide sequence; Parvovirus; parvovirus infection; priority journal; serodiagnosis; substance abuse; Taiwan; virus gene; Adult; Aged; Cloning, Molecular; DNA, Viral; Female; HIV Infections; HIV-1; Humans; Immunoblotting; Immunoglobulin G; Immunoglobulin M; Longitudinal Studies; Male; Middle Aged; Parvoviridae Infections; Parvovirus; Polymerase Chain Reaction; Recombinant Fusion Proteins; Substance Abuse, Intravenous; SUMO-1 Protein; Taiwan; Young Adultjournal article10.1016/j.jcv.2011.01.017213536292-s2.0-79953741941