2017-06-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/707172This project has been arranged for three years, and the specific aims are as follows: First year: To investigate the permeability of different anti-bacterial and anti- inflammation drugs by using the transwell dentin disc model. The results found that Lysine Acetylsalicylate, Diclofenac Na, Ampicillin sodium, Ginsenoside Rg1 were selected for the second year experiment. Second year: To investigate cytotoxicity of the anti-bacterial and anti-inflammation. Detection of cell viability, it is of great biocompatibility that Lysine Acetylsalicylate / Amp., Ginsenoside Rg1 / Amp. To evaluate the anti-bacterial activity of drugs against S. aureus, E. faecalis and E. coli. However, there was no difference between groups in the diameter of the inhibition zone. Furthermore, it was evaluated whether anti-bacterial and anti-inflammatory drugs had an effect on the osteogenesis differentiation of human dental pulp cells (HDPSCs). The results showed that Lysine Acetylsalicylate and Ginsenoside Rg1 had the same effect as the control group. Third year: According to the previous study, the combination of anti-bacterial and anti-inflammation drugs with better permeability and biocompatibility, BSP/Amp., Lysine Acetylsalicylate/Amp.and Ginsenoside Rg1/Amp., will be selected. (1) To induce inflammation, Lipopolysaccharides (LPS) and Lipoteichoic acid (LTA) are applied to dental pulp stem cells (DPSC). The releasing of inflammatory cytokines is detected and the anti-inflammatory and anti-bacterial effects of drugs are also evaluated. (2) Drugs with the better anti-inflammatory and anti-bacterial effects are selected to apply in animal study. (3) Beagle dog animal model with experimental pulpitis will be established. (4) CCMS /H3PO4 was applied after the drugs penetrated through dentinal tubules, and the crystallization and occlusion performance in dentinal tubules will also be evaluated. (5) Indirect and direct pulp capping are performed to compare the anti-inflammatory and anti-bacterial effects of the selected drugs to traditional pulp capping materials on dentin-pulp complex in an animal model. (6) To observe the ability of CCMS / H3PO4 preparations to occlude dentin tubules by SEM. (7) To investigate the dynamic precipitation of CCMS / H3PO4 preparations by Sub-μ-CT analysis.