Adav S.S.Lee D.-J.Wang A.Ren N.2019-05-132019-05-13200909608524https://scholars.lib.ntu.edu.tw/handle/123456789/407763A functional bacterial consortium that can effectively hydrolyze cellobiose and produce bio-hydrogen was isolated by a concentration-to-extinction approach. The sludge from a cattle feedlot manure composting plant was incubated with 2.5-20 g l-1 cellobiose at 35 ¢XC and pH 6.0. The microbial diversity of serially concentrated suspensions significantly decreased following increasing cellobiose concentration, finally leaving only two viable strains, Clostridium butyricum strain W4 and Enterococcus saccharolyticus strain. This consortium has a maximum specific hydrogen production rate of 2.19 mol H2 mol hexose-1 at 5 g l-1 cellobiose. The metabolic pathways shifted from ethanol-type to acetate-butyrate type as cellobiose concentration increased from 2.5 to >7 g l-1. The concentration-to-extinction approach is effective for isolating functional consortium from natural microflora. In this case the functional strains of interest are more tolerant to the increased loadings of substrates than the non-functional strains. ? 2008 Elsevier Ltd. All rights reserved.CellobioseFunctional consortiaHydrogenHydrolysis[SDGs]SDG7Bacterial consortiums; Bio hydrogens; Cattle feedlots; Cellobiose; Clostridium butyricum; Concentrated suspensions; Functional consortia; Functional strains; Metabolic pathways; Microbial diversities; Microflora; Non-functional; Specific hydrogen production rates; Ethanol; Gas fuel manufacture; Gas producers; Hydrogen; Hydrolysis; Manures; Nonmetals; Strain; Hydrogen production; acetic acid; alcohol; butyric acid; cellobiose; hexose; hydrogen; bacterium; biofuel; composting; hydrogen; hydrolysis; manure; metabolism; pH; sludge; substrate; sugar; article; bacterial strain; cattle; Clostridium butyricum; composting; Enterococcus; hydrolysis; incubation time; manure; metabolism; microbial diversity; microflora; nonhuman; nucleotide sequence; pH; priority journal; sludge; Animals; Cattle; Cellobiose; Clostridium butyricum; Enterococcus; Hydrogen; Hydrogen-Ion Concentration; Manure; Sewage; Temperature; Bacteria (microorganisms); Bos; Clostridium butyricum; Enterococcus saccharolyticusjournal article10.1016/j.biortech.2008.12.0142-s2.0-59649127998https://www.scopus.com/inward/record.uri?eid=2-s2.0-59649127998&doi=10.1016%2fj.biortech.2008.12.014&partnerID=40&md5=e2ccfd13005688aaafaa56042d3a340d