JANG-JAER LEEChang Y.-L.Lai W.-L.JENG-YUH KOYEN-PING KUOCHUN-PIN CHIANGAzuma M.Chen C.-W.JEAN-SAN CHIA2021-07-052021-07-0520111043-3074https://www.scopus.com/inward/record.uri?eid=2-s2.0-80051765765&doi=10.1002%2fhed.21607&partnerID=40&md5=882d9cc6fe0ea57896dca1c73e056e0fhttps://scholars.lib.ntu.edu.tw/handle/123456789/569174Background. T helper 17 (Th17) and regulatory T cells share plasticity in the expression of interleukin (IL)-17 and forkhead box P3 (FOXP3), but their mutual presence in human diseases is unclear. Methods. IL-17 and FOXP3 were analyzed by immunohistostaining and flow cytometry. The cytokine milieu was analyzed by quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Results. Oral squamous cell carcinoma expresses high levels of IL-1β, IL-6, and transforming growth factor (TGF)-β. A unique subset of FOXP3 + IL-17-producing CD4 + T cells was consistently identified in tumor-infiltrating lymphocytes from advanced stages of cancer, but not in the circulation, at a frequency of 0.5% to 5.5 % of total CD4 + T and positively correlated with the frequency of IL-17 +FOXP3 - T cells. The IL-17 +FOXP3 + T cells express CCR6 and suppress the proliferation of autologous CD4 + CD25 - responder T-cells in vitro. Conclusions. The prevalence of IL-17-producing FOXP3 + CD4 + tumor infiltrating lymphocytes is increased in oral squamous cell carcinoma. ? 2010 Wiley Periodicals, Inc. Head Neck, 2010 Copyright ? 2010 Wiley Periodicals, Inc.[SDGs]SDG3chemokine receptor CCR6; interleukin 17; interleukin 1beta; interleukin 6; transcription factor FOXP3; transforming growth factor beta; adult; aged; article; cancer staging; CD25+ T lymphocyte; CD4+ T lymphocyte; clinical article; controlled study; female; flow cytometry; gingiva; human; human cell; human tissue; immunohistochemistry; lymphocyte proliferation; male; mouth carcinoma; prevalence; priority journal; protein analysis; reverse transcription polymerase chain reaction; squamous cell carcinoma; tumor associated leukocyte; Adult; Aged; Carcinoma, Squamous Cell; Case-Control Studies; CD4-Positive T-Lymphocytes; Female; Flow Cytometry; Forkhead Transcription Factors; Humans; Immunohistochemistry; Interleukin-17; Interleukin-1beta; Interleukin-6; Lymphocytes, Tumor-Infiltrating; Male; Middle Aged; Mouth Neoplasms; Prevalence; Receptors, CCR6; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Transforming Growth Factor betajournal article10.1002/hed.21607218377002-s2.0-80051765765