2012-02-142024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/661384摘要：植物菌質體 (phytoplasma)屬於Mollicutes綱之不具細胞壁 (cell wall-less) 的革蘭氏陽性 (Gram positive) 細菌，且為絕對寄生性的病原菌 (obligate parasite)，侷限生長在植物韌皮部 (phloem) 中，是一種影響寄主植物生長之重要植物病原細菌，常造成簇葉 (witches’ broom）、花器綠化 (virescence) 葉化 (phyllody)、黃化 (yellowing) 及小葉 (little leaf) 等病徵，於自然界中主要由葉蟬 (leafhopper)、木蝨 (Psyllid) 等媒介昆蟲在植物間傳播。植物菌質體目前尚無法利用人工培養，因此其基因組成及生理生化特性仍無法做全盤深入之瞭解。全世界目前已發現之植物菌質體種類多達28群，可在數百種植物上造成1000多種病害。現今在植物菌質體之診斷或鑑定時，必須先進行16S rDNA解序及演化樹建構之繁瑣流程後方可確認植物菌質體之身分，所需時間也相當長，本計畫之目的在於針對檢疫上各群重要之植物菌質體，如aster yellows (16SrI group), X-disease (16SrIII group)等，利用 in silico RFLP技術建立檢疫用分子資訊。另利用質體鑑別性序列，針對分屬各群或亞群植物菌質體之地理或寄主菌系(strain)進行鑑別，開發新穎之診斷鑑定技術，完成並整合上述各階段快速與精確之診斷鑑定流程，以達到快速鑑別植物菌質體之目的，以提供進口種苗、種球、糧作與花卉等植物及其產品檢疫之有效偵檢技術，以有效防堵檢疫有害生物入侵，確保國內農業生產安全。<br> Abstract: Phytoplasmas, former mycoplasma-like organisms (MLOs) can cause many yellows-type diseases. In Taiwan and other countries, there are more than 1000 different plant diseases are now known to be caused by over 800 phytoplasma species. Up to now, the phytopathogenic phytoplasmas still resist to be cultured in any available medium. It`s also very difficult to purify phytoplasmas from affected plants without the contamination of plant antigens. The biological and biochemical data of phytoplasmas are also next to nothing. In this proposed study, the in silico RFLP databases of 16Sr group I, III , XII phytoplasmas will be set up and the phytoplasma specific PCR primers based on the plasmid sequences will also be developed. The in silico RFLP analysis and PCR-based detection strategies thus developed will be useful for the differentiation of different groups, subgroups, species, or even strains or isolates of various important quarantine phytoplasmas. The detection system will also provide a useful tool for plant inspection and quarantine authorities and researchers to deal with phytoplasma diseases.植物菌質體電腦模擬限制&#37238片段長度多型性質體群 亞群菌系phytoplasmain silico RFLPplasmidgroupsubgroupstrain