Abstract
摘要:本計劃主要目的是針對一個多功能之微型全分析系統進行研究開發,預期由法方開發一使用於化學與生醫檢驗系統之螢光微粒子與感測器,再由此一微系統分析螢光微粒子的濃度,且可對此粒子進行操控與合成。本計畫乃是基於台法雙方的研究專長,利用微機電製程的方式,製作一系列不同幾何尺寸的微流道,並由法方提供各相關分子的化學特性,配合法方已具備之共軛焦顯微鏡量測技術與本實驗室之微粒子顯像測速儀系統,量測不同特性的化學粒子在微通道流場中的運動情形,再配合微電極陣列所提供之介電泳力,設計一多功能電極陣列晶片以作為微粒子操控的平台,結合法方為生醫系統應用開發之各種不同特性的化學分子,可望開發一完整微分析系統,達成研究目的。
目前本實驗室已具備微粒子影像測速儀系統的研究能量,並已完成其測試,另外相關微機電製程技術,可製作出一系列不同幾何尺寸的微流道。第一年的研究目標為利用非對稱擋體式無閥門微幫浦,開發具有主動混合功能的壓電式微混合器,預定在微流道中設置不對稱擋體,當壓電片的震動會推動流體流經不對稱擋體,由於混合器內部為一非定常往復流流場,在擋體週遭的流場會產生迴流區,且此迴流區會隨著壓電片的振動頻率而消長,因此可利用此一現象加以設計,成為一個具有推動流體功能的主動式混合器。
第二年有二個工作項目,首先將配合第一年的成果,以微粒子顯像測速儀來量測電式微混合器內的流場,以驗證效能,並配合量測法方所開發的同軸微流場系統,為第三年的系統整合做準備。其次則是經由粒子運動與介電泳力效應之間的交互作用,設計出介電泳粒子操控晶片,配合法方於第二年所開發之螢光微粒子,預期將可選擇性的達成粒子集中或分離等各種不同的操作結果,以縮短分析時間並增加精確度。
第三年則將配合前二年的成果,上半年的重點是由台方開發一應用於同軸流微流場之微粒子操控晶片,法方則是開發螢光微粒子的感測器,下半年則是針對壓電式微混合器、同軸流粒子操控晶片與螢光粒子感測器進行一系統整合,配合理論分析並結合相關影像處理技術將實驗結果量化,以對此一多功能型化學與生醫之微分析系統的研究開發獲得成果。
本研究的成果將對於化學與生醫之微分析系統的建立有直接的貢獻,尤其本研究將以法方所開發之螢光粒子作為檢測,此粒子之預期目標將可直接與待檢驗細胞相結合,對於諸醫系統的檢驗、藥物傳輸等相關應用有極大幫助。除了對微流體力學學理上之基礎研究將有所貢獻外,亦可對生醫晶片中微系統之設計有所助益。
Abstract: The present proposed research project is to be a jointed research program between Taiwan and France. The objectives are aiming at having the scientific and technical expertise of both laboratories in the microfluidics field to develop several micro-devices. These devices can be used for the analytical detection of ionic or neutral species as well as the synthesis and manipulation of micro-nanoparticles for the chemical or biological analysis.
In many research fields like environmental or biological analysis, there is a need for miniaturized systems, very often called “micro-system for total analysis” , or -TAS, or “lab-on-a-chip”. The first micro-device that we propose to build is a microfluidic device for the fluorimetric titration of cations or neutral molecules of interest in the field of environmental or biological analysis. This device would include a PZT valveless micropump by MEMS technique, an active or passive micromixer, and a detection part. Detection of the species of interest will be made by fluorescence analysis in a first step and micro-cantilever deformation in a second step. The first method is appropriate for many cations detection and the second method is more appropriate for detection of biological species, such as sugars, peptides and proteins.
The developments of -TAS or lab-on-a-chip have been substantially becoming important issues during the past few years. However, along with this development, the techniques to manipulate particles in a micro-fluidic system are not successfully obtained all along. This study is also to develop a particle-manipulating technique by dielectrophoresis force used for the molecular transport in a micro-fluidic system. Moreover, there is often a need to select particles from liquids according to differences in their mass or in their sizes. In order to do this separation, we propose to use a dielectrophoretic effect in order to control the moving of particles, and their collection and separation. This implies the manufacturing of an integrated microcircuit with electrodes conveniently located inside a microchamber or a microchannel.
In this study, in order to obtain the flow characteristics without any interference to the flow field, an optical method by using micro Particle-Image-Velocimetry (Micro-PIV) will be set up for the specific flow measurements. Therefore, the velocity information of the particle manipulating and the flow in non-circular micro-channels can be obtained. Furthermore, in order to increase the signal-to-noise ratio, the image of different size particles will be processed independently.
Keyword(s)
微型全分析系統
微粒子顯像測速儀
微粒子操控
介電
micro-TAS
dielectrophoretic
particle-manipulating
Micro-PIV