https://scholars.lib.ntu.edu.tw/handle/123456789/136521
Title: | Establishment of a fluorescence resonance energy transfer-based bioassay for detecting dioxin-like compounds | Authors: | Lin, Chi-Iou Hsieh, Chun-Hung Lee, Stanley Shiao-Ying Lee, Wei-Shan Chang-Chien, Gou-Ping Pan, Chien-Yuan Lee, Hsinyu |
Issue Date: | 2008 | Journal Volume: | 15 | Journal Issue: | 6 | Start page/Pages: | 833-840 | Source: | Journal of Biomedical Science | Abstract: | Dioxins comprise a group of compounds which contain a double aromatic ring-like structure. They are among the most prevalent and toxic environmental pollutants. Accumulation of dioxins in human tissues poses a potential threat to human health. Currently, analytical chemical procedures dominate dioxin-detection protocols. In this study, we established a fluorescence resonance energy transfer (FRET)-based dioxin-detection bioassay. Aryl hydrocarbon receptor (AHR) and AHR nuclear translocator (ARNT) fused-cyan fluorescent protein (CFP) and -yellow fluorescent protein (YFP) constructed were transiently co-transfected into rat hepatoma cell line, H4IIEC3 cells. Our results showed that no FRET signals were detected in AHR-CFP- and ARNT-YFP-transfected H4IIEC3 cells. However, dioxin treatments upregulated FRET signals in these transfected cells in a dose-dependent manner. This work highlighted the potential of FRET technique in the detection of dioxin-like compounds. ? 2008 National Science Council Taipei. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/161584 http://ntur.lib.ntu.edu.tw/bitstream/246246/161584/1/31.pdf |
DOI: | 10.1007/s11373-008-9267-6 | SDG/Keyword: | 2,3,7,8 tetrachlorodibenzo para dioxin; aromatic hydrocarbon receptor; cyan fluorescent protein; dioxin; hypoxia inducible factor 1beta; yellow fluorescent protein; agar gel electrophoresis; animal cell; article; bioassay; cancer cell culture; controlled study; fluorescence resonance energy transfer; hepatoma cell; nonhuman; plasmid; priority journal; rat; upregulation; Animals; Aryl Hydrocarbon Receptor Nuclear Translocator; Biological Assay; Cell Line, Tumor; Dioxins; Dose-Response Relationship, Drug; Fluoresceins; Fluorescence Resonance Energy Transfer; Rats; Receptors, Aryl Hydrocarbon; Teratogens; Tetrachlorodibenzodioxin; Rattus |
Appears in Collections: | 生命科學系 |
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