https://scholars.lib.ntu.edu.tw/handle/123456789/160032
標題: | Exon Selection in -Tropomyosin Mrna Is Regulated by the Antagonistic Action of Rbm4 and Ptb | 作者: | LIN, JUNG-CHUN TARN, WOAN-YUH |
公開日期: | 2005 | 卷: | v.25 | 期: | n.22 | 起(迄)頁: | 10111-10121 | 來源出版物: | MOLECULAR AND CELLULAR BIOLOGY | 摘要: | RNA-binding motif protein 4 (RBM4) has been implicated in the regulation of precursor mRNA splicing. Using differential display analysis, we identified mRNAs that associate with RBM4-containing messenger RNPs in vivo. Among these mRNAs, -tropomyosin (-TM) is known to exhibit a muscle cell type-specific splicing pattern. The level of the skeletal muscle- specific -TM mRNA isoform partially correlated with that of RBM4 in human tissues examined and could be modulated by ectopic overexpression or suppression of RBM4. These results indicated that RBM4 directly influences the expression of the skeletal muscle-specific - TM isoform. Using minigenes, we demonstrated that RBM4 can activate the selection of skeletal muscle-specific exons, possibly via binding to intronic pyrimidine-rich elements. By contrast, the splicing regulator polypyrimidine tract binding protein (PTB) excluded these exons; moreover, RBM4 antagonized this PTB-mediated exon exclusion likely by competing with PTB for binding to a CU-rich element. This study suggests a possible mechanism underlying the regulated alternative splicing of -TM by the antagonistic splicing regulators RBM4 and PTB. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/92458 |
顯示於: | 分子醫學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。