|Title:||Establishment and Characterization of a Human Gastric Carcinoma Cell Line Tmc-1||Authors:||SHYU, RONG-YAUN
|Keywords:||gastric cancer;cell line;interferon;karyotypic aberration||Issue Date:||2004||Source:||CELLS TISSUES ORGANS||Journal Volume:||v.177||Journal Issue:||n.1||Start page/Pages:||37-46||Abstract:||
Established cancer cell lines are useful in the study of various cancers. We established a human gastric carcinoma cell line TMC-1 derived from the lymph node of a moderately differentiated adenocarcinoma of the stomach. TMC-1 cells grew in vitro as a mixture of attached and suspension cells, and exhibited spindle or ovoid morphology. They had a population doubling time of 15 h, a plating efficiency of 61 %, formed colonies in semisolid agar, secreted the tumor marker CA 19-9, and were tumorigenic in athymic nude mice. The cells expressed E-cadherin and beta-catenin. The karyotypic analysis demonstrated hyperdiploid features with a modal chromosome of 53 . The cell had the deletion at chromosome 18q and gains at chromosome 2p13 -25, 5p15, 5q21- 35, 7, 8q24, 9q, 11, 12p, 14q24-32 and 20. Analysis by fluorescence in situ hybridization showed the deletion at 7 qtel and duplication at 7q11.2 at the rearranged chromosome 7. Growth of TMC-1 cells was inhibited by 27-32% by interferon-alpha (2,000 U/ml) and by interferon-gamma with an IC50 of 125 U/ml. The cell line is tumorigenic in vivo, and its growth is moderately inhibited by interferon-alpha and interferon-gamma. It can be used to develop new modalities of human gastric cancer treatment. Copyright (C) 2004 S. Karger AG, Basel.
|Appears in Collections:||實驗動物中心|
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