L aser-induced fluorescence technique for DNA and proteins separated by capillary electrophoresis

Abstract

Recent developments in capillary electrophoresis (CE) in conjunction with laser-induced fluorescence (LIF) using long-wavelength (maximum excitation wavelength.500 nm) dyes are reviewed. These dyes are particularly of interest when conducting the analyses of biopolymers by CE–LIF using He–Ne lasers. These systems are benefited from low background, low costs, easy maintenance, & compactness. Derivatizations of DNA & proteins with fluorescent or nonfluorescent chemicals can be carried out prior to, during, or after separations. With the advantages of sensitivity, rapidity, & high efficiency, the applications of CE–LIF to the analysis of polymerase chain reaction products, DNA sequencing, trace analysis of proteins, & single cell analysis have been presented.