https://scholars.lib.ntu.edu.tw/handle/123456789/177693
標題: | L aser-induced fluorescence technique for DNA and proteins separated by capillary electrophoresis | 作者: | Lin, Yang-Wei Chiu, Tai-Chia Chang, Huan-Tsung |
關鍵字: | Reviews;DNA;Proteins | 公開日期: | 2003 | 出版社: | Taipei:National Taiwan University Dept Chem Engn | 起(迄)頁: | - | 來源出版物: | Journal of Chromatography B | 摘要: | Recent developments in capillary electrophoresis (CE) in conjunction with laser-induced fluorescence (LIF) using long-wavelength (maximum excitation wavelength.500 nm) dyes are reviewed. These dyes are particularly of interest when conducting the analyses of biopolymers by CE–LIF using He–Ne lasers. These systems are benefited from low background, low costs, easy maintenance, & compactness. Derivatizations of DNA & proteins with fluorescent or nonfluorescent chemicals can be carried out prior to, during, or after separations. With the advantages of sensitivity, rapidity, & high efficiency, the applications of CE–LIF to the analysis of polymerase chain reaction products, DNA sequencing, trace analysis of proteins, & single cell analysis have been presented. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/2006111501275593 | 其他識別: | 246246/2006111501275593 |
顯示於: | 化學系 |
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