https://scholars.lib.ntu.edu.tw/handle/123456789/314752
標題: | Gene expression profiles associated with response to chemotherapy in epithelial ovarian cancers | 作者: | ERIC YAO-YU CHUANG | 公開日期: | 九月-2005 | 來源出版物: | Clinical Cancer Research | 摘要: | Purpose: The goal of this study was to determine whether distinct gene expression profiles are associated with intrinsic and/or acquired chemoresistance in epithelial ovarian carcinoma. Experimental Design: Gene expression profiles were generated from 21 primary chemosensitive tumors and 24 primary chemoresistant tumors using cDNA-based microarrays. Gene expression profiles of both groups of primary tumors were then compared with those of 15 ovarian carcinomas obtained following platinum-based chemotherapy ("postchemotherapy" tumors). A theme discovery tool was used to identify functional categories of genes involved in drug resistance. Results: Comparison of primary chemosensitive and chemoresistant tumors revealed differential expression of 85 genes (P < 0.001). Comparison of gene expression profiles of primary chemo-sensitive tumors and postchemotherapy tumors revealed more robust differences with 760 genes differentiating the two groups (P < 0.001). In contrast, only 230 genes were differentially expressed between primary chemoresistant and postchemotherapy groups (P < 0.001). Common to both gene lists were 178 genes representing transcripts differentially expressed between post-chemotherapy tumors and all primary tumors irrespective of intrinsic chemosensitivity. The gene expression profile of postchemotherapy tumors compared with that of primary tumors revealed statistically significant overrepresentation of genes encoding extracellular matrix-related proteins. Conclusions: These data show that gene expression profiling can discriminate primary chemo-resistant from primary chemosensitive ovarian cancers. Gene expression profiles were also identi-fied that correlate with states of intrinsic and acquired chemoresistance and that represent targets for future investigation and potential therapeutic interventions. © 2005 American Association for Cancer Research. |
URI: | http://scholars.lib.ntu.edu.tw/handle/123456789/314752 https://www.scopus.com/inward/record.uri?eid=2-s2.0-24344485096&doi=10.1158%2f1078-0432.CCR-04-2682&partnerID=40&md5=b2b2645f6fa3897e3faa08c8de13299c |
ISSN: | 10780432 | DOI: | 10.1158/1078-0432.CCR-04-2682 | SDG/關鍵字: | carboplatin; cisplatin; cyclophosphamide; gene product; paclitaxel; platinum derivative; scleroprotein; adult; aged; article; cancer chemotherapy; chemosensitivity; DNA microarray; drug resistance; drug response; female; gene expression profiling; human; human tissue; major clinical study; ovary carcinoma; postchemotherapy tumor; priority journal; Adult; Aged; Aged, 80 and over; Antineoplastic Agents; Carcinoma, Endometrioid; Cystadenocarcinoma, Serous; Female; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Humans; Immunoenzyme Techniques; Middle Aged; Neoplasm Proteins; Neoplasm Staging; Oligonucleotide Array Sequence Analysis; Ovarian Neoplasms; Reverse Transcriptase Polymerase Chain Reaction; RNA, Neoplasm; Tumor Markers, Biological |
顯示於: | 生醫電子與資訊學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。