https://scholars.lib.ntu.edu.tw/handle/123456789/317065
標題: | Improved Gene Expression by a Modified Bicistronic Retroviral Vector | 作者: | Hsieh C.-L. Chen B.-F. Wang C.-C. Liu H.-H. DING-SHINN CHEN Hwang L.-H. |
公開日期: | 1995 | 卷: | 214 | 期: | 3 | 起(迄)頁: | 910-917 | 來源出版物: | Biochemical and Biophysical Research Communications | 摘要: | We have previously described the construction of a bicistronic retroviral vector using the picornavirus internal ribosome entry site (IRES), which allows two genes expression simultaneously from a single transcript. This vector transcribes RNA efficiently; however, in some cases the levels of protein production are low. In this report, we further modified the bicistronic vector by abolishing the functional viral gag initiation codon that is retained in the vector at 5′ to the first initiation codon of transduced gene. Five different genes, human interleukin 2 (hIL-2), human interleukin 4 (hIL-4), human granulocyte macrophage stimulating factor (hGM-CSF), herpes simplex virus thymidine kinase (HSV-tk) gene, and hepatitis C virus (HCV) core gene (C190), were tested on this modified vector for gene transfer and expression. Our results demonstrated that the new bicistronic vector greatly increased the protein levels when compared with the original one. As the RNA levels and splicing patterns from these two vectors remained similar, the improvement was most likely resulted from the increased translational efficiency. ? 1995 Academic Press. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028828210&doi=10.1006%2fbbrc.1995.2373&partnerID=40&md5=243078e30cc7131a169bc4bacf890eba http://scholars.lib.ntu.edu.tw/handle/123456789/317065 |
DOI: | 10.1006/bbrc.1995.2373 | SDG/關鍵字: | core protein; granulocyte macrophage colony stimulating factor; interleukin 2; interleukin 4; messenger rna; thymidine kinase; virus enzyme; animal cell; article; cistron; cloning vector; controlled study; dna recombination; gene expression regulation; gene transfer; hepatitis c virus; herpes simplex virus; human; human cell; nonhuman; priority journal; protein synthesis; rat; retrovirus; rna splicing; translation regulation; Animal; Blotting, Western; Carcinoma, Hepatocellular; Cell Line; Enzyme-Linked Immunosorbent Assay; Gene Expression; Genes, Structural; Genetic Vectors; Glioma; Granulocyte-Macrophage Colony-Stimulating Factor; Hela Cells; Hepacivirus; Human; Interleukin-2; Interleukin-4; Kinetics; Liver Neoplasms; Rats; Recombinant Proteins; Retroviridae; Simplexvirus; Support, Non-U.S. Gov't; Thymidine Kinase; Transfection; Tumor Cells, Cultured; Viral Core Proteins |
顯示於: | 醫學系 |
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