https://scholars.lib.ntu.edu.tw/handle/123456789/330508
標題: | Antrodia cinnamomea fruiting bodies extract suppresses the invasive potential of human liver cancer cell line PLC/PRF/5 through inhibition of nuclear factor κB pathway | 作者: | Hsu, Y.-L. Kuo, P.-L. Cho, C.-Y. Ni, W.-C. Tzeng, T.-F. Ng, L.-T. Kuo, Y.-H. Lin, C.-C. LEAN-TEIK HUANG |
關鍵字: | Antrodia cinnamomea; Invasion; Liver cancer; MMPs; NF-κB | 公開日期: | 2007 | 卷: | 45 | 期: | 7 | 起(迄)頁: | 1249-1257 | 來源出版物: | Food and Chemical Toxicology | 摘要: | In this study, we first report the anti-invasive effect of ethylacetate extract from Antrodia cinnamomea (EAC) fruiting bodies in the human liver cancer cell line PLC/PRF/5. Treatment with EAC decreased the cancer invasion of PLC/PRF/5 cells in a dose-dependent manner. This effect was strongly associated with a concomitant decrease in either the level or activity of VEGF, MMP-2, MMP-9 and MT1-MMP, and an increase in the expression of TIMP-1 and TIMP-2. EAC inhibited constitutively activated and inducible NF-κB in both its DNA-binding activity and transcriptional activity. Furthermore, EAC also inhibited the TNF-α-activated NF-κB-dependent reporter gene expression of MMP-9 and VEGF, and the invasion of cancer cells. EAC also exhibited an inhibitory effect on angiogenesis in a Matrigel Plug Angiogenesis Assay. Further investigation revealed that EAC's inhibition of cancer cell growth and invasion was also evident in a nude mice model. Our results indicate that EAC inhibits the activation of NF-κB, and may provide a molecular basis for drug development using EAC as an anti-invasive agent in the prevention and treatment of cancer. ? 2007 Elsevier Ltd. All rights reserved. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-34247163539&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/330508 |
DOI: | 10.1016/j.fct.2007.01.005 | SDG/關鍵字: | acetic acid ethyl ester; Antrodia cinnamomea extract; gelatinase A; gelatinase B; immunoglobulin enhancer binding protein; matrigel; matrix metalloproteinase 14; natural product; tissue inhibitor of metalloproteinase 1; tissue inhibitor of metalloproteinase 2; tumor necrosis factor alpha; unclassified drug; vasculotropin; angiogenesis; animal experiment; animal model; antineoplastic activity; Antrodia cinnamomea; article; cancer cell culture; cancer inhibition; cancer invasion; cancer model; controlled study; drug DNA binding; fruiting body; genetic transcription; human; human cell; male; mouse; nonhuman; nude mouse; Acetates; Antineoplastic Agents, Phytogenic; Carcinoma, Hepatocellular; Cell Line, Tumor; Dose-Response Relationship, Drug; Drug Screening Assays, Antitumor; Fruiting Bodies, Fungal; Humans; Liver Neoplasms; Matrix Metalloproteinase 14; Matrix Metalloproteinase 2; Matrix Metalloproteinase 9; Neoplasm Invasiveness; NF-kappa B; Plant Extracts; Polyporales; Solvents; Tissue Inhibitor of Metalloproteinase-1; Tissue Inhibitor of Metalloproteinase-2; Tumor Markers, Biological; Vascular Endothelial Growth Factor A; Antrodia cinnamomea; Mus musculus |
顯示於: | 農業化學系 |
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