https://scholars.lib.ntu.edu.tw/handle/123456789/356837
標題: | Chlorophyll a, an active anti-proliferative compound of Ludwigia octovalvis, activates the CD95 (APO-1/CD95) system and AMPK pathway in 3T3-L1 cells | 作者: | Wu, S.-J. Ng, L.-T. Wang, G.-H. Huang, Y.-J. Chen, J.-L. Sun, F.-M. LEAN-TEIK HUANG |
關鍵字: | 3T3-L1 cells; AMPK; CD95 (APO-1/CD95); Chlorophyll a; Ludwigia octovalvis | 公開日期: | 2010 | 卷: | 48 | 期: | 2 | 起(迄)頁: | 716-721 | 來源出版物: | Food and Chemical Toxicology | 摘要: | Ludwigia octovalvis is an aquatic plant widely distributed in Taiwan. It is traditionally used as a diuretic and is consumed as health drink. In this study, we evaluated the anti-proliferative activity of extracts and active constituent (chlorophyll a; CHL-a) of L. octovalvis in 3T3-L1 adipocytes; its mode of action on apoptosis was also investigated. Results showed that, among the different extracts and fractions, the ethylacetate layer (EAL) possessed the most potent anti-proliferative activity. Activity guided fractionation of the EAL obtained the bioactive constituent CHL-a (IC50: 24.10 ± 0.83 nM). At concentrations 5-30 nM, CHL-a exhibited a dose-dependent accumulation of the Sub-G1 peak and caused cell cycle arrest at the G0/G1 phase. At 30 nM, it significantly reduced the cell viability, induced the appearance of DNA fragments, and enhanced the activation of caspase-3. Western blot data revealed that CHL-a decreased the level of Bcl-2, and increased the expression of CD95 (APO-1/CD95) and Bax. Furthermore, CHL-a up-regulated the AMPK and p-AMPK levels, and down-regulated the expression of PPAR-γ. These results conclude that CHL-a possesses potent anti-proliferative activity, and its apoptotic effects on 3T3-L1 adipocytes are mediated through the activation of CD95 (APO-1/CD95) system and the AMPK signaling pathway. ? 2009 Elsevier Ltd. All rights reserved. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-74649083924&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/356837 |
DOI: | 10.1016/j.fct.2009.12.001 | SDG/關鍵字: | caspase 3; chlorophyll; Fas ligand; ludwigia octovalvis extract; mitogen activated protein kinase; plant extract; protein Bax; protein bcl 2; unclassified drug; adipocyte; animal cell; apoptosis; article; cell cycle arrest; cell cycle G0 phase; cell cycle G1 phase; cell cycle progression; cell growth; cell population; cell proliferation; cell viability; controlled study; DNA fragmentation; down regulation; enzyme activation; fractionation; gene expression regulation; Ludwigia octovalvis; medicinal plant; nonhuman; protein expression; signal transduction; upregulation; Western blotting; 3T3-L1 Cells; Adipocytes; Animals; Anti-Obesity Agents; Antigens, CD95; Apoptosis; Cell Cycle; Cell Proliferation; Chemical Fractionation; Chlorophyll; Mice; Onagraceae; Plant Extracts; PPAR gamma; Protein Kinases; Ludwigia octovalvis |
顯示於: | 農業化學系 |
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