https://scholars.lib.ntu.edu.tw/handle/123456789/374188
Title: | Myocyte Shape Regulates Lateral Registry of Sarcomeres and Contractility | Authors: | PO-LING KUO Hyungsuk Lee Mark-Anthony Bray Nicholas A. Geisse Yen-Tsung Huang William J. Adams Sean P. Sheehy Kevin K. Parker |
Issue Date: | Dec-2012 | Journal Volume: | 181 | Journal Issue: | 6 | Start page/Pages: | 2030-2037 | Source: | American Journal of Pathology | Abstract: | The heart actively remodels architecture in response to various physiological and pathological conditions. Gross structural change of the heart chambers is directly reflected at the cellular level by altering the morphological characteristics of individual cardiomyocytes. However, an understanding of the relationship between cardiomyocyte shape and the contractile function remains unclear. By using in vitro assays to analyze systolic stress of cardiomyocytes with controlled shape, we demonstrated that the characteristic morphological features of cardiomyocytes observed in a variety of pathophysiological conditions are correlated with mechanical performance. We found that cardiomyocyte contractility is optimized at the cell length/width ratio observed in normal hearts, and decreases in cardiomyocytes with morphological characteristics resembling those isolated from failing hearts. Quantitative analysis of sarcomeric architecture revealed that the change of contractility may arise from alteration of myofibrillar structure. Measurements of intracellular calcium in myocytes revealed unique characteristics of calcium metabolism as a function of myocyte shape. Our data suggest that cell shape is critical in determining contractile performance of single cardiomyocytes by regulating the intracellular structure and calcium handling ability. © 2012 American Society for Investigative Pathology. |
URI: | http://scholars.lib.ntu.edu.tw/handle/123456789/374188 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84869171054&doi=10.1016%2fj.ajpath.2012.08.045&partnerID=40&md5=aea6ccb4e06a8def19373ed3cfb72d7e |
ISSN: | 00029440 | DOI: | 10.1016/j.ajpath.2012.08.045 | SDG/Keyword: | calcium; animal cell; animal tissue; article; calcium cell level; calcium metabolism; cell shape; cell stress; controlled study; correlation analysis; heart muscle cell; heart muscle contractility; in vitro study; muscle fibril; nonhuman; priority journal; quantitative analysis; rat; sarcomere; systole; Animals; Calcium; Cell Shape; Diastole; DNA; Image Processing, Computer-Assisted; Myocardial Contraction; Myocytes, Cardiac; Rats; Rats, Sprague-Dawley; Sarcomeres; Systole |
Appears in Collections: | 電機工程學系 |
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