https://scholars.lib.ntu.edu.tw/handle/123456789/377942
標題: | Suppression of adipogenesis and obesity in high-fat induced mouse model by hydroxylated polymethoxyflavones | 作者: | Lai, C.-S. Ho, M.-H. Tsai, M.-L. Li, S. Badmaev, V. Ho, C.-T. MIN-HSIUNG PAN |
關鍵字: | 3T3-L1; adenosine monophosphate-activated protein kinase (AMPK); high-fat diet (HFD); hydroxylated polymethoxyflavones (HPMFs); obesity | 公開日期: | 2013 | 卷: | 61 | 期: | 43 | 起(迄)頁: | 10320-10328 | 來源出版物: | Journal of Agricultural and Food Chemistry | 摘要: | This study demonstrated that hydroxylated polymethoxyflavones (HPMFs) effectively and dose-dependently suppressed accumulation of lipid droplets in adipocytes by approximately 51-55%. Western blot analysis revealed that HPMFs markedly down-regulated adipogenesis-related transcription factors peroxisome proliferator-activated receptor (PPAR) γ and sterol regulatory element-binding protein (SREBP)-1c as well as downstream target fatty acid binding protein 2 (aP2), fatty acid synthase (FAS), and acetyl-CoA carboxylase (ACC). In addition, HPMFs also activated adenosine monophosphate-activated protein kinase (AMPK) signaling in 3T3-L1 adipocytes. In the early phase of adipogenesis, HPMF-treated preadipocytes displayed a delayed cell cycle entry into G2/M phase at 24 h (35.5% for DMI group and 4.8% for 20 μg/mL HPMFs-treated group) after initiation of adipogenesis. Furthermore, administration of HPMFs (0.25 and 1%) decreased high-fat diet (HFD) induced weight gain (15.3 ± 3.9 g for HFD group, 10.3 ± 0.3 g and 7.9 ± 0.7 g for 0.25 and 1% HPMFs groups, respectively) and relative perigonadal, retroperitoneal, mesenteric fat weight in C57BL/6 mice. Administration of HPMFs reduced serum levels of aspartate aminotransferase (GOT), alanine aminotransferase (GPT), triglycerides (TG), and total cholesterol (T-cho). The results suggested that HPMFs may have a potential benefit in preventing obesity. ? 2013 American Chemical Society. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84887076166&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/377942 |
DOI: | 10.1021/jf402257t | SDG/關鍵字: | 3T3-L1; High-fat diet; obesity; Polymethoxyflavones; Protein kinase; Amino acids; Cytology; Fatty acids; Hydroxylation; Mammals; Proteins; Nutrition; acetyl coenzyme A carboxylase; antiobesity agent; flavone derivative; peroxisome proliferator activated receptor gamma; plant extract; 3T3 cell line; adipocyte; adipogenesis; animal; C57BL mouse; chemistry; Citrus; conference paper; cytology; disease model; down regulation; drug effect; fruit; genetics; human; lipid diet; male; metabolism; mouse; obesity; pathophysiology; 3T3-L1 Cells; Acetyl-CoA Carboxylase; Adipocytes; Adipogenesis; Animals; Anti-Obesity Agents; Citrus; Diet, High-Fat; Disease Models, Animal; Down-Regulation; Flavones; Fruit; Humans; Male; Mice; Mice, Inbred C57BL; Obesity; Plant Extracts; PPAR gamma |
顯示於: | 食品科技研究所 |
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