https://scholars.lib.ntu.edu.tw/handle/123456789/384177
標題: | Development of CpG-Oligodeoxynucleotides for effective activation of rabbit TLR9 mediated immune responses | 作者: | LI-CHUNG HSU Chuang, Tsung-Hsien Lai, Chao-Yang Tseng, Ping-Hui Yuan, Chiun-Jye Hsu, Li-Chung Ploegh, Hidde L. |
公開日期: | 2014 | 卷: | 9 | 期: | 9 | 來源出版物: | PLoS ONE | 摘要: | CpG-oligodeoxynucleotides (CpG-ODN) are potent immune stimuli being developed for use as adjuvants in different species. Toll-like receptor 9 (TLR9) is the cellular receptor for CpG-ODN in mammalian cells. The CpG-ODN with 18-24 deoxynucleotides that are in current use for human and mouse cells, however, have low activity with rabbit TLR9. Using a cell-based activation assay, we developed a type of CpG-ODN containing a GACGTT or AACGTT motif in 12 phosphorothioate-modified deoxynucleotides with potent stimulatory activity for rabbit TLR9. The developed CpG-ODN have higher activities than other developed CpG-ODN in eliciting antigen-nonspecific immune responses in rabbit splenocytes. When mixed with an NJ85 peptide derived from rabbit hemorrhagic disease virus, they had potent activities to boost an antigen-specific T cell activation and antibody production in rabbits. Compared to Freund's adjuvant, the developed CpG-ODN are capable of boosting a potent and less toxic antibody response. The results of this study suggest that both the choice of CpG-motif and its length are important factors for CpG-ODN to effectively activate rabbit TLR9 mediated immune responses. Copyright: ? 2014 Chuang et al. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84907486110&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/384177 |
DOI: | 10.1371/journal.pone.0108808 | SDG/關鍵字: | adenine; CpG oligodeoxynucleotide; cytosine; guanine; NJ85 peptide; phosphorothioic acid; thymine; toll like receptor 9; unclassified drug; virus protein; CPG-oligonucleotide; cytokine; immunoglobulin M; immunological adjuvant; oligodeoxyribonucleotide; peptide; phosphate; phosphorodithioic acid; toll like receptor 9; adjuvant therapy; animal cell; antibody production; antigen specificity; Article; controlled study; drug efficacy; drug potency; drug safety; immunostimulation; Lagovirus; mouse; nonhuman; nucleotide motif; spleen cell; T lymphocyte activation; toxicity testing; animal; chemistry; drug effects; HEK293 cell line; human; immunology; lymphocyte activation; metabolism; nucleotide sequence; rabbit; Rabbit hemorrhagic disease virus; spleen; T lymphocyte; Oryctolagus cuniculus; Adjuvants, Immunologic; Animals; Antibody Formation; Base Sequence; Cytokines; HEK293 Cells; Hemorrhagic Disease Virus, Rabbit; Humans; Immunoglobulin M; Lymphocyte Activation; Oligodeoxyribonucleotides; Peptides; Phosphates; Rabbits; Spleen; T-Lymphocytes; Toll-Like Receptor 9 |
顯示於: | 分子醫學研究所 |
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