https://scholars.lib.ntu.edu.tw/handle/123456789/392502
標題: | Antroquinonol from Antrodia Camphorata suppresses breast tumor migration/invasion through inhibiting ERK-AP-1- and AKT-NF-κB-dependent MMP-9 and epithelial-mesenchymal transition expressions | 作者: | Lee, Wai-Theng TZONG-HUEI LEE Cheng, Chia-Hsiung Chen, Ku-Chung Chen, Yen-Chou Lin, Cheng-Wei |
關鍵字: | Antroquinonol; EMT; Invasion; MMP-9; TPA | 公開日期: | 2015 | 卷: | 78 | 起(迄)頁: | 33 - 41 | 來源出版物: | Food and Chemical Toxicology | 摘要: | Antroquinonol (ANQ) is an ubiquinon derivative isolated from the mycelium of Antrodia camphorata. However, the effect of ANQ on breast cancer treatment is unknown. We found that ANQ significantly suppressed the migration and invasion of breast cancer MDA-MB-231 cells, and inhibited 12-. O-tetradecanoylphorbol-13-acetate (TPA)-induced invasiveness by MCF7 cells. ANQ inhibiting MMP-9 gene expression and enzymatic activity occurred at transcriptional regulation. Mechanistically, activation of ERK and AKT is crucial for MMP-9 gene expression, and the addition of ANQ suppressed phosphorylation of ERK and AKT. The induction of the AP-1 and NF-κB pathway participated in MMP-9 gene expression. Suppression of ERK inhibited AP-1, whereas blocking AKT diminished NF-κB activity, and treatment with ANQ suppressed both AP-1 and NF-κB signaling. Moreover, ANQ suppressed EMT protein expression, and inhibited TPA-induced EMT through downregulating the ERK-AP-1 and AKT-NF-κB signaling cascades. Together, our data showed for the first time that ANQ inhibited breast cancer invasiveness by suppressing ERK-AP-1- and AKT-NF-κB-dependent MMP-9 and EMT expressions. ? 2015 Elsevier Ltd. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84922502678&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/392502 |
ISSN: | 02786915 | DOI: | 10.1016/j.fct.2015.01.012 | SDG/關鍵字: | antineoplastic agent; antroquinonol; gelatinase B; immunoglobulin enhancer binding protein; mitogen activated protein kinase; protein kinase B; transcription factor AP 1; unclassified drug; antroquinonol; gelatinase B; immunoglobulin enhancer binding protein; mitogen activated protein kinase 3; MMP9 protein, human; phorbol 13 acetate 12 myristate; transcription factor AP 1; ubiquinone; Article; breast cancer cell line; cancer cell destruction; cancer cell line; cancer inhibition; cell invasion; cell migration; cell viability; controlled study; down regulation; drug structure; epithelial mesenchymal transition; gene expression regulation; human; human cell; MCF 7 cell line; MDA MB 231 cell line; metastasis; MMP 9 gene; protein expression; signal transduction; tumor invasion; upregulation; analogs and derivatives; antagonists and inhibitors; Antrodia; breast tumor; cell motion; cell survival; chemistry; drug effects; epithelial mesenchymal transition; genetics; metabolism; pathology; phosphorylation; tumor cell line; Taiwanofungus camphoratus; Antrodia; Breast Neoplasms; Cell Line, Tumor; Cell Movement; Cell Survival; Down-Regulation; Epithelial-Mesenchymal Transition; Humans; Matrix Metalloproteinase 9; MCF-7 Cells; Mitogen-Activated Protein Kinase 3; NF-kappa B; Phosphorylation; Signal Transduction; Tetradecanoylphorbol Acetate; Transcription Factor AP-1; Ubiquinone |
顯示於: | 漁業科學研究所 |
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