https://scholars.lib.ntu.edu.tw/handle/123456789/393223
標題: | In vitro gene expression profile of bovine peripheral blood mononuclear cells in early mycobacterium bovis infection | 作者: | Cheng, Yafen CHUNG-HSI CHOU HSIANG-JUNG TSAI |
關鍵字: | Global gene expression; Immune response; Macrophage; Mycobacterium bovis | 公開日期: | 2015 | 卷: | 10 | 期: | 6 | 起(迄)頁: | 2102-2118 | 來源出版物: | Experimental and Therapeutic Medicine | 摘要: | The intracellular parasite Mycobacterium bovis (M. bovis) causes tuberculosis in cattle and humans. Understanding the interactions between M. bovis and host cells is essential in developing tools for the prevention, detection, and treatment of M. bovis infection. Gene expression profiles provide a large amount of information regarding the molecular mechanisms underlying these interactions. The present study analyzed changes in gene expression in bovine peripheral blood mononuclear cells (PBMCs) at 0, 4 and 24 h following exposure to M. bovis. Using bovine whole-genome microarrays, a total of 420 genes were identified that exhibited significant alterations in expression (?2-fold). Significantly enriched genes were identified using the Kyoto Encyclopedia of Genes and Genomes database, of which the highest differentially expressed genes were associated with the immune system, signal transduction, endocytosis, cellular transport, inflammation, and apoptosis. Of the genes associated with the immune system, 84.85% displayed downregulation. These findings support the view that M. bovis inhibits signaling pathways of antimycobacterial host defense in bovine PBMCs. These in vitro data demonstrated that molecular alterations underlying the pathogenesis of tuberculosis begin early, during the initial 24 h following M. bovis infection. ? 2015, Spandidos Publications. All rights reserved. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84946544214&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/393223 |
DOI: | 10.3892/etm.2015.2814 | SDG/關鍵字: | animal cell; apoptosis; Article; bovine; bovine tuberculosis; cell transport; controlled study; down regulation; endocytosis; female; gene expression; immune system; inflammation; microarray analysis; Mycobacterium bovis; nonhuman; peripheral blood mononuclear cell; polymerase chain reaction; signal transduction; spectrophotometry; upregulation |
顯示於: | 獸醫學系 |
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