https://scholars.lib.ntu.edu.tw/handle/123456789/402418
標題: | Boswellia serrata resin extract alleviates azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced colon tumorigenesis | 作者: | Chou, Y.-C. Suh, J.H. Wang, Y. Pahwa, M. Badmaev, V. Ho, C.-T. MIN-HSIUNG PAN |
關鍵字: | Azoxymethane; Boswellia Serrata; Colorectal cancer; Dextran sulfate sodium; Inflammation | 公開日期: | 2017 | 卷: | 61 | 期: | 9 | 來源出版物: | Molecular Nutrition and Food Research | 摘要: | Scope: Boswellia serrata (BS) resin is a popular dietary supplement for joint nourishment. In this study, we investigated the chemopreventive effects of dietary BS extract and its impact of gut microbiota on azoxymethane/dextran sulfate sodium (AOM/DSS)-induced colitis-associated colon cancer in mice. Methods and results: Male ICR mice were injected with AOM and 2% DSS via drinking water. The mice were fed with 0.25 or 0.5% BS extract, and colonic tissue were collected at 15 weeks. The main effective components of BS supercritical CO2 extraction were analyzed by LC-MS/MS are boswellic acids. We found that treatment with BS extract significantly reduce the colonic tumor formation. Western blot and histological analysis revealed that dietary BS extract could markedly reduce the inflammation associated protein levels expression. Furthermore, BS extract reduced cell proliferation via inhibiting phosphorylation level of protein kinase B (Akt), glycogen synthase kinase 3β (GSK3β), and downregulation of cyclin D1. In addition, BS extract also altered the composition of gut microbiota by enhancing the proportion of Clostridiales and reducing the percentage of Bacteroidales. Conclusion: In summary, BS extract decreased the protein levels of inflammative enzymes such as inducible nitric oxide synthase and cyclooxygenase-2 in colonic mucosa. It also mediated Akt/GSK3β/cyclin D1 signaling pathway and altered the composition of gut microbiota to alleviate tumor growth. Taken together, this study suggests that BS extract has great potential to suppress colon tumorigenesis. ? 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-85016932759&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/402418 |
DOI: | 10.1002/mnfr.201600984 | SDG/關鍵字: | azoxymethane; Ccnd1 protein, mouse; cyclin D1; dextran sulfate; glycogen synthase kinase 3beta; plant extract; plant resin; protein kinase B; animal; antagonists and inhibitors; Boswellia; cell proliferation; chemically induced; colon tumor; drug effect; Institute for Cancer Research mouse; male; MAPK signaling; mouse; pathology; Animals; Azoxymethane; Boswellia; Cell Proliferation; Colonic Neoplasms; Cyclin D1; Dextran Sulfate; Glycogen Synthase Kinase 3 beta; Male; MAP Kinase Signaling System; Mice; Mice, Inbred ICR; Plant Extracts; Proto-Oncogene Proteins c-akt; Resins, Plant |
顯示於: | 食品科技研究所 |
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