https://scholars.lib.ntu.edu.tw/handle/123456789/413594
標題: | Effects of garlic oil and its organosulfur compounds on the activities of hepatic drug-metabolizing and antioxidant enzymes in rats fed high- and low-fat diets | 作者: | Sheen L.-Y. Chen H.-W. Kung Y.-L. Liu C.-T. Lii C.-K. |
公開日期: | 1999 | 卷: | 35 | 期: | 2 | 起(迄)頁: | 160-166 | 來源出版物: | Nutrition and Cancer | 摘要: | We examined the effects of garlic oil (GO) and two of its organosulfur compounds, diallyl sulfide (DAS) and diallyl disulfide (DADS), on the drug- metabolizing and antioxidant systems in rats and sought to determine whether these effects are associated with dietary fat. Rats were fed a high-fat diet and received GO or DADS (200 mg/kg body wt) or DAS (100 mg/kg) orally three times a week for seven weeks. Control animals received corn oil alone. Another group of rats was fed a low-fat diet, with or without GO. GO and DADS significantly reduced the body weight gain of rats (p <0.05). GO, however, dramatically increased the spleen weight and spleen weight-to-body weight ratio (p < 0.05). DAS increased glutathione S-transferase (GST) and 7- pentoxyresorufin O-dealkylase activities, whereas DADS increased only GST activity (p<0.05). Immunoblot assay showed GO-, DAS-, and DADS-enhanced expression of the placental form of GST and cytochrome P-450 IIBI but suppressed cytochrome P-450 IIEI expression. Hepatic antioxidant enzyme activities were also modulated by these garlic components. GO and DADS inhibited glutathione peroxidase activity (p<0.05), and DADS and DAS enhanced glutathione reductase activity (p<0.05). Only GO enhanced the superoxide dismutase activity (p<0.05). All these garlic components increased glutathione levels in red blood cells (p<0.05) but did not influence hepatic glutathione levels. Although the amount of fat in the diet modulated drug- metabolizing and antioxidant functions, no interactions between GO and dietary fat were observed. These results indicate that GO and its allyl sulfide components, as well as dietary lipid, modulate drug-metabolizing and antioxidant enzyme activities. The action of GO appears to be independent of dietary lipid content. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/413594 | ISSN: | 1635581 | DOI: | 10.1207/S15327914NC352_10 |
顯示於: | 食品科技研究所 |
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