https://scholars.lib.ntu.edu.tw/handle/123456789/414030
標題: | Structurally characterized arabinogalactan from Anoectochilus formosanus as an immuno-modulator against CT26 colon cancer in BALB/c mice | 作者: | Yang L.-C. Hsieh C.-C. Lu T.-J. Lin W.-C. |
關鍵字: | Anoectochilus formosanus;Anti-cancer;Arabinogalactan;Immuno-modulator;Immunotherapy;Polysaccharide | 公開日期: | 2014 | 卷: | 21 | 期: | 5 | 起(迄)頁: | 647-655 | 來源出版物: | Phytomedicine | 摘要: | In this study, the innate immuno-modulatory effects and anti-cancer action of arabinogalactan (AG), a derivative of a well-known orchid, Anoectochilus formosanus, were investigated. The innate immuno-modulatory effects of AG were determined in vitro using RAW 264.7 cells for microarray analysis, and in vivo using BALB/c mice administrated with AG at 5 and 15 mg/kg intra-peritoneally for 3 weeks. The anti-cancer activity of AG was evaluated by CT26 colon cancer-bearing BALB/c mice. The microarray analysis was performed to evaluate the innate immunity and demonstrated that AG significantly induced the expression of cytokines, chemokines, and co-stimulatory receptors, such as IL-1£\, CXCL2, and CD69. An intraperitoneal injection of AG in mice increased the spleen weight, but not the body weight. The treatment of mitogen, LPS significantly stimulated splenocyte proliferation in AG treated groups. The AG treatment also promoted splenocyte cytotoxicity against YAC-1 cells and increased the percentage of CD3+CD8+ cytotoxic T cells in innate immunity test. Our experiments revealed that AG significantly decreased both tumour size and tumour weight. Besides, AG increased the percentage of DC, CD3+CD8+ T cells, CD49b+CD3- NK cells among splenocytes, and cytotoxicity activity in tumour-bearing mice. In addition, the immunohistochemistry of the tumour demonstrated that the AG treatments increased the tumour-filtrating NK and cytotoxic T-cell. These results demonstrated that AG, a polysaccharide derived from a plant source, has potent innate immuno-modulatory and anti-cancer activity. AG may therefore be used for cancer immunotherapy. ? 2013 Elsevier GmbH. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/414030 | ISSN: | 09447113 | DOI: | 10.1016/j.phymed.2013.10.032 | SDG/關鍵字: | arabinogalactan; CD69 antigen; chemokine; CXCL2 chemokine; cytokine; fluorouracil; interleukin 1alpha; mitogenic agent; polysaccharide; arabinogalactan; galactan; immunologic factor; plant extract; animal experiment; animal model; Anoectochilus formosanus; antineoplastic activity; article; Bagg albino mouse; body weight; cancer cell culture; cancer immunotherapy; cell proliferation; clinical evaluation; colon cancer; controlled study; cytotoxic T lymphocyte; human; immunohistochemistry; immunomodulation; innate immunity; medicinal plant; microarray analysis; mouse; nonhuman; priority journal; protein expression; spleen cell; spleen weight; treatment duration; animal; carcinoma; chemistry; Colonic Neoplasms; drug effects; drug screening; gene expression profiling; isolation and purification; macrophage; male; metabolism; Orchidaceae; phytotherapy; randomization; spleen; Anoectochilus formosanus; Mus; Animals; Body Weight; Carcinoma; Colonic Neoplasms; Drug Screening Assays, Antitumor; Galactans; Gene Expression Profiling; Immunity, Innate; Immunologic Factors; Macrophages; Male; Mice, Inbred BALB C; Orchidaceae; Phytotherapy; Plant Extracts; Random Allocation; Spleen |
顯示於: | 食品科技研究所 |
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