https://scholars.lib.ntu.edu.tw/handle/123456789/417194
標題: | Isolation of a chromosomal region of Klebsiella pneumoniae associated with allantoin metabolism and liver infection | 作者: | Chou H.-C. Lee, Cha-Ze Ma L.-C. CHI-TAI FANG SHAN-CHWEN CHANG JIN-TOWN WANG |
公開日期: | 2004 | 卷: | 72 | 期: | 7 | 起(迄)頁: | 3783-3792 | 來源出版物: | Infection and Immunity | 摘要: | Klebsiella pneumoniae liver abscess with metastatic complications is an emerging infectious disease in Taiwan. To identify genes associated with liver infection, we used a DNA microarray to compare the transcriptional profiles of three strains causing liver abscess and three strains not associated with liver infection. There were 13 clones that showed higher RNA expression levels in the three liver infection strains, and 3 of these 13 clones contained a region that was absent in MGH 78578. Sequencing of the clones revealed the replacement of 149 bp of MGH 78578 with a 21,745-bp fragment in a liver infection strain, NTUH-K2044. This 21,745-bp fragment contained 19 open reading frames, 14 of which were proven to be associated with allantoin metabolism. The K2044)(ΔallS) mutant showed a significant decrease of virulence in intragastric inoculation of BALB/c mice, and the prevalence of this chromosomal region was significantly higher in strains associated with liver abscess than in those that were not (19 or 32 versus 2 of 94; P = 0.0001 [χ2 test]). Therefore, the 22-kb region may play a role in K. pneumoniae liver infection and serve as a marker for rapid identification. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-3042692195&doi=10.1128%2fIAI.72.7.3783-3792.2004&partnerID=40&md5=d6b8c929633b24160c5b4de040d2df3d https://scholars.lib.ntu.edu.tw/handle/123456789/417194 |
ISSN: | 0019-9567 | DOI: | 10.1128/IAI.72.7.3783-3792.2004 | SDG/關鍵字: | allantoin; animal experiment; animal model; article; bacterial strain; bacterial virulence; bacterium isolation; chromosome isolation; controlled study; DNA microarray; female; gene expression; genetic association; hepatitis; human; inoculation; Klebsiella pneumoniae; liver abscess; metabolism; mouse; nonhuman; nucleotide sequence; priority journal; sequence analysis; Allantoin; Amino Acid Sequence; Animals; Base Sequence; Female; Infection; Klebsiella pneumoniae; Liver; Liver Abscess; Mice; Molecular Sequence Data; Oligonucleotide Array Sequence Analysis; RNA, Messenger |
顯示於: | 微生物學科所 |
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