https://scholars.lib.ntu.edu.tw/handle/123456789/426015
Title: | Manufacture characterization of anti-inflammatory liposomes from jumbo flying squid (Dosidicus gigas) skin phospholipid extraction | Authors: | Yi-Feng Kao Yi-Hsieng Samuel Wu CHUNG-HSI CHOU Shih-Guei Fu Cheng-Wei Liu Huey-Jine Chai YI-CHEN CHEN |
Issue Date: | 2018 | Journal Volume: | 9 | Start page/Pages: | 3986-3996 | Source: | Food & Function | Abstract: | The anti-inflammation properties of marine phospholipids enriched with n-3 fatty acids contribute to anti-inflammatory and inflammation-resolving mediators. Functional squid-skin (SQ) liposomes were manufactured from squid-skin phospholipids, and their anti-inflammatory effects were investigated. SQ liposomes included phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylethanolamine (PE), phosphatidylcholine (PC), and lysophosphatidylcholine (Lyso-PC), and had an approximate diameter of 100 mm. When RAW264.7 cells were treated with the SQ liposome, no (p > 0.05) cytotoxicity was observed below a concentration of 7.5 mg mL-1. An SQ-liposome pretreatment of lipopolysaccharide (LPS)-induced RAW 264.7 cells showed decreased (p < 0.05) prostaglandin E2 (PGE2), nitric oxide (NO), interleukin-1beta (IL-1β), IL-6, and tumor necrosis factor-alpha (TNF-α). The engulfment of SQ liposomes by the RAW264.7 cells resulted in lower (p < 0.05) LPS-induced intracellular levels of reactive oxygen species. Furthermore, an SQ-liposome administration ameliorated (p < 0.05) carrageenan-induced paw edema in mice. SQ liposomes may act via apoptotic mimicry to elicit the resolution of inflammation and prevent chronic inflammation-related diseases. ? The Royal Society of Chemistry. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/426015 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050554229&doi=10.1039%2fc8fo00767e&partnerID=40&md5=1cd4ce7ff71c8757d9114013796bcb1f |
DOI: | 10.1039/c8fo00767e | SDG/Keyword: | Fatty acids; Molluscs; Nitric oxide; Pathology; Phospholipids; Shellfish; Anti-inflammatory effects; Chronic inflammation; Intracellular levels; Lysophosphatidyl-choline; Phosphatidylethanolamine; Phosphatidylinositol; Reactive oxygen species; Tumor necrosis factor alpha; Liposomes; antiinflammatory agent; immunoglobulin enhancer binding protein; interleukin 1beta; liposome; nitric oxide; phospholipid; prostaglandin E2; tumor necrosis factor; animal; C57BL mouse; chemistry; Decapodiformes; drug effect; edema; genetics; human; immunology; isolation and purification; macrophage; male; mouse; RAW 264.7 cell line; skin; Animals; Anti-Inflammatory Agents; Decapodiformes; Dinoprostone; Edema; Humans; Interleukin-1beta; Liposomes; Macrophages; Male; Mice; Mice, Inbred C57BL; NF-kappa B; Nitric Oxide; Phospholipids; RAW 264.7 Cells; Skin; Tumor Necrosis Factor-alpha |
Appears in Collections: | 動物科學技術學系 |
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