https://scholars.lib.ntu.edu.tw/handle/123456789/431067
標題: | Immunofluorescence assay for detection of the nucleocapsid antigen of the severe acute respiratory syndrome (SARS)-associated coronavirus in cells derived from throat wash samples of patients with SARS | 作者: | Liu I.-J. PEI-JER CHEN Shiou-Hwei Yeh Chiang Y.-P. LI-MIN HUANG MING-FU CHANG SHEY-YING CHEN PAN-CHYR YANG SHAN-CHWEN CHANG Wang W.-K. |
公開日期: | 2005 | 出版社: | American Society for Microbiology | 卷: | 43 | 期: | 5 | 起(迄)頁: | 2444-2448 | 來源出版物: | Journal of Clinical Microbiology | 摘要: | An antigen detection assay for severe acute respiratory syndrome (SARS) coronavirus was established in this study by an indirect immunofluorescence test, which utilized cells derived from throat wash samples of patients with SARS and a rabbit serum that recognized the nucleocapsid protein of SARS-associated coronavirus (SARS-CoV) but not that of other human coronavirus tested. It detected SARS-CoV in 11 of 17 (65%) samples from SARS patients as early as day 2 of illness but in none of the 10 samples from healthy controls. Compared with other diagnostic modalities for detecting SARS-CoV, this assay is simpler, more convenient, and economical. It could be an alternative for early and rapid diagnosis, should SARS return in the future. Copyright ? 2005, American Society for Microbiology. All Rights Reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84983724699&doi=10.1128%2fJCM.43.5.2444-2448.2005&partnerID=40&md5=a964a2eb326a465bc903cd663fec51ac https://scholars.lib.ntu.edu.tw/handle/123456789/431067 |
DOI: | 10.1128/JCM.43.5.2444-2448.2005 | SDG/關鍵字: | nucleocapsid protein; adult; animal cell; antigen detection; antigen recognition; article; clinical article; controlled study; cost benefit analysis; early diagnosis; human; immunofluorescence test; intermethod comparison; lavage; nonhuman; priority journal; reverse transcription polymerase chain reaction; SARS coronavirus; serum; severe acute respiratory syndrome; throat; Coronavirus; human coronavirus; Oryctolagus cuniculus; SARS associated coronavirus; SARS CoV |
顯示於: | 生物化學暨分子生物學科研究所 |
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