Immunofluorescence assay for detection of the nucleocapsid antigen of the severe acute respiratory syndrome (SARS)-associated coronavirus in cells derived from throat wash samples of patients with SARS
Journal
Journal of Clinical Microbiology
Journal Volume
43
Journal Issue
5
Pages
2444-2448
Date Issued
2005
Author(s)
Liu I.-J.
Chiang Y.-P.
Wang W.-K.
Abstract
An antigen detection assay for severe acute respiratory syndrome (SARS) coronavirus was established in this study by an indirect immunofluorescence test, which utilized cells derived from throat wash samples of patients with SARS and a rabbit serum that recognized the nucleocapsid protein of SARS-associated coronavirus (SARS-CoV) but not that of other human coronavirus tested. It detected SARS-CoV in 11 of 17 (65%) samples from SARS patients as early as day 2 of illness but in none of the 10 samples from healthy controls. Compared with other diagnostic modalities for detecting SARS-CoV, this assay is simpler, more convenient, and economical. It could be an alternative for early and rapid diagnosis, should SARS return in the future. Copyright ? 2005, American Society for Microbiology. All Rights Reserved.
SDGs
Other Subjects
nucleocapsid protein; adult; animal cell; antigen detection; antigen recognition; article; clinical article; controlled study; cost benefit analysis; early diagnosis; human; immunofluorescence test; intermethod comparison; lavage; nonhuman; priority journal; reverse transcription polymerase chain reaction; SARS coronavirus; serum; severe acute respiratory syndrome; throat; Coronavirus; human coronavirus; Oryctolagus cuniculus; SARS associated coronavirus; SARS CoV
Publisher
American Society for Microbiology
Type
journal article