https://scholars.lib.ntu.edu.tw/handle/123456789/444448
標題: | Acrylamide-induced mitochondria collapse and apoptosis in human astrocytoma cells | 作者: | Chen J.-H. Yang C.-H. Wang Y.-S. JIANN-GWU LEE Cheng C.-H. CHIN-CHENG CHOU |
公開日期: | 2013 | 卷: | 51 | 期: | 1 | 起(迄)頁: | 446-452 | 來源出版物: | Food and Chemical Toxicology | 摘要: | Acrylamide (ACR) can be produced during food processing and has neurotoxic effects in humans. This study aims to determine ACR induced apoptotic responses in human astrocytoma U-1240 MG cells to realize the incurred toxic mechanisms. Under 1 and 2mM ACR exposure, cell viability decreased as time increased. The increments in sub-G1 phase were 87.5-fold, and pro-caspase 3 and PARP protein expressions decreased 35% and 54.5% respectively relative to the control after 2mM ACR treatment. Molecular evidence of Bax/bcl-2 ratio and cytochrome c expression increased 8.86-fold and 6.81-fold as well as pro-caspase 9 decreased 67.8% relative to the control respectively under 2mM ACR exposure. Trolox, an ROS scavenging agent, attenuated cell death and induced ROS production by 2mM ACR. The ultrastructure alterations of mitochondria showed marked vesicular matrix compartmentalization and cytoplasmic vacuole formation after 2mM ACR was treated for 48h, whereas those treated for 72h showed chromatin condensation, pyknosis, and swelling. These results indicate long-term exposure to ACR induced mitochondria collapse and finally led to apoptosis. Although 2mM ACR is higher than average daily intake dosage, workers in chemical industries may be exposed to sufficient doses to entail health risks. ? 2012 Elsevier Ltd. |
URI: | https://www.scopus.com/record/display.uri?eid=2-s2.0-84870559730&origin=resultslist&sort=plf-f&cite=2-s2.0-84870559730&src=s&imp=t&sid=f3f291fb832634321bf70f66c82c779c&sot=cite&sdt=a&sl=0 | ISSN: | 0278-6915 | DOI: | 10.1016/j.fct.2012.10.025 | SDG/關鍵字: | acrylamide; cytochrome c; nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase; procaspase 3; procaspase 9; protein BAD; protein Bax; protein bcl 2; reactive oxygen metabolite; trolox C; apoptosis; article; astrocytoma cell; cell compartmentalization; cell cycle arrest; cell cycle G1 phase; cell swelling; cell ultrastructure; cell vacuole; cell viability; cellular parameters; chemical industry; chromatin condensation; concentration response; controlled study; cytoplasm; DNA repair; drug cytotoxicity; flow cytometry; health hazard; human; human cell; in vitro study; incubation time; long term exposure; mitochondrial collapse; mitochondrion; occupational exposure; oxidation reduction state; oxidative stress; protein analysis; protein expression; pyknosis; transmission electron microscopy; Western blotting; Acrylamide; Apoptosis; Astrocytoma; bcl-2-Associated X Protein; Caspase 3; Cell Line, Tumor; Cell Survival; Chromans; Cytochromes c; Dose-Response Relationship, Drug; G1 Phase; Humans; Membrane Potential, Mitochondrial; Mitochondria; Poly(ADP-ribose) Polymerases; Reactive Oxygen Species; Toxicity Tests |
顯示於: | 獸醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。