https://scholars.lib.ntu.edu.tw/handle/123456789/445793
標題: | Destroying activity of glycine coated magnetic nanoparticles on lysozyme, alpha-lactalbumin, insulin and alpha-crystallin amyloid fibrils | 作者: | Antosova, Andrea Bednarikova, Zuzana Koneracka, Martina Antal, Iryna Zavisova, Vlasta Kubovcikova, Martina Wu, Josephine W. Wang, Steven S.-S. Gazova, Zuzana STEVEN SHENG-SHIH WANG |
關鍵字: | Amyloid fibrils; Insulin; Lysozyme; Magnetic nanoparticles; α-Crystallin; α-Lactalbumin | 公開日期: | 2019 | 卷: | 471 | 起(迄)頁: | 169-176 | 來源出版物: | Journal of Magnetism and Magnetic Materials | 摘要: | A great variety of human protein deposition and protein aggregation diseases (Alzheimer's disease, diabetes mellitus, cataract, systemic amyloidosis and other) have been associated with the accumulation of amyloid fibrils in different tissues. Therefore, development of the agents able to reduce amyloid deposits represents an attractive strategy for their treatment. We have investigated ability of glycine coated magnetic nanoparticles (Gly-MNPs) to destroy protein amyloid fibrils. The properties of Gly-MNPs were characterized with the aim to identify the optimized conditions for the glycine adsorption on surface of MNPs. It was found that Gly-MNPs have superparamagnetic behavior and their size, isoelectric point and stability depend on the amount of the glycine in the samples. The obtained results suggest that optimal weight ratio (w/w) for the modification of MNPs by glycine (Gly/Fe3O4) is equal to 5/1. The selected Gly5-MNPs1 were used for the study of their effect on amyloid fibrils of four globular proteins, namely lysozyme, bovine α-lactalbumin, insulin and α-crystallin. It was found that Gly5-MNPs1 destroy lysozyme, α-lactalbumin and insulin amyloid fibrils in concentration dependence manner. However, Gly5-MNPs1 were not able significantly destroy bovine α-crystallin amyloid fibrils. We assume that obtained results represent important contribution for rational design of potential therapeutics of amyloid diseases based on nanoparticles. ? 2018 Elsevier B.V. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/445793 | ISSN: | 0304-8853 | DOI: | 10.1016/j.jmmm.2018.09.096 | SDG/關鍵字: | Amino acids; Enzymes; Insulin; Mammals; Nanomagnetics; Nanoparticles; Neurodegenerative diseases; Amyloid fibril; Attractive strategies; Concentration dependence; Crystallin; Insulin amyloid fibrils; Magnetic nano-particles; Protein aggregation disease; Superparamagnetic behavior; Glycoproteins |
顯示於: | 化學工程學系 |
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