https://scholars.lib.ntu.edu.tw/handle/123456789/452463
Title: | Amino acid sequence of trichoanguina, a ribosomal-inactivating protein from Trichosanthes anguina seeds | Authors: | LU-PING CHOW Kamo M. Lin J.-Y. Wang S.-H. Ueno Y. Tsugita A. |
Keywords: | Protein sequence; Ribosome-inactivating protein(s); Trichoanguina; Trichosanthes anguina | Issue Date: | 1996 | Journal Volume: | 3 | Journal Issue: | 3 | Start page/Pages: | 178-186 | Source: | Journal of Biomedical Science | Abstract: | In this study, we sequenced a new type I ribosome-inactivating protein, trichoanguina, from the seeds of Trichosanthes anguina (snake gourd). Trichoanguina is a basic glycoprotein having an apparent molecular mass of 35.0 kD and possessing strong ribosome-inactivating activity. Trichoanguina was cleaved with cyanogen bromide and partially digested with thermolysin, chymotrypsin, trypsin and Staphylococcus aureus V8 protease. The subsequent peptide fragments were separated by SDS-polyacrylamide gel electrophoresis, followed by electroblotting to polyvinylidene difluoride membranes and then sequencing. The sequencing of trichoanguina was completed, consisting of 245 amino acid residues. The sequencing of trichoanguina revealed a considerable homology to trichosanthin and α-trichosanthin, which are known as abortifacient, ribosome-inactivating and antihuman immunodeficiency virus proteins, with 46.7% and 55.6% amino acid identities, respectively. The sequence conserves two active sites: Glu- 158 and Arg- 161. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029778772&partnerID=40&md5=5deaf6de89ba32648854a9ff580aea90 https://scholars.lib.ntu.edu.tw/handle/123456789/452463 |
ISSN: | 10217770 | SDG/Keyword: | abortive agent; anti human immunodeficiency virus agent; chymotrypsin; cyanogen bromide; glycoprotein; ribosome inactivating protein; thermolysin; trichoanguina; Trichosanthes extract; trichosanthin; trypsin; unclassified drug; amino acid sequence; article; nonhuman; plant seed; priority journal; protein degradation; sequence homology; Anguina; Cucurbita; Human immunodeficiency virus; Serpentes; Staphylococcus aureus; Trichosanthes; Trichosanthes cucumerina var. anguina |
Appears in Collections: | 生物化學暨分子生物學科研究所 |
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