https://scholars.lib.ntu.edu.tw/handle/123456789/452628
標題: | Hyperglycemia and advanced glycation end products (AGEs) suppress the differentiation of 3T3-L1 preadipocytes | 作者: | Chang, Chia-Chu Chen, Chen-Yu GEEN-DONG CHANG Chen, Ting-Huan Chen, Woan-Ling Wen, Hui-Chin Huang, Chih-Yang Chang, Chung-Ho |
關鍵字: | Adipogenesis; Advanced glycation end products (AGEs); Bcl-2; Hyperglycemia; NF-kB | 公開日期: | 2017 | 卷: | 8 | 期: | 33 | 起(迄)頁: | 55039 - 55050 | 來源出版物: | Oncotarget | 摘要: | Aging is characterized by mild hyperglycemia and accumulation of advanced glycation end products (AGEs). Effects of chronic exposure to hyperglycemia or AGEs on the adipogenic differentiation of 3T3-L1 preadipocytes remain unclear. We examined the chronic effect of AGEs and high glucose on the differentiation of 3T3-L1 cells by culturing 3T3-L1 cells in the presence of AGEs or 25 mM glucose for 1 month. Chronic incubation of 3T3-L1 cells with AGEs or high glucose blocked their differentiation into mature adipocytes as evidenced by reduced levels of adipocyte markers such as accumulated oil droplets, GPDH, aP2, adiponectin and of adipogenesis regulators PPARγ and C/EBPα. Levels or activities of Src, PDK1, Akt, and NF-κB were higher in AGEs- and high glucose-treated cells than those in 3T3-L1 cells. Levels of Bcl-2 were elevated in AGEs- and high glucose-treated cells, and were attenuated by inhibitors of PI3-kinase, Akt and NF-κB. Moreover, adipogenesis was attenuated in 3T3-L1 cells stably expressing Bcl-2 or YAP. These results suggest that chronic AGEs and high glucose treatments up-regulate Bcl-2 and YAP via the Akt-NF-κB pathway and impair adipogenesis. ? Chang et al. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/452628 https://www2.scopus.com/inward/record.uri?eid=2-s2.0-85029085760&doi=10.18632%2foncotarget.18993&partnerID=40&md5=3505f65f1dcfbec43f8258c974ac1ee0 |
ISSN: | 19492553 | DOI: | 10.18632/oncotarget.18993 | SDG/關鍵字: | adiponectin; advanced glycation end product; glucose; immunoglobulin enhancer binding protein; peroxisome proliferator activated receptor gamma; phosphatidylinositol 3 kinase; phosphoinositide dependent protein kinase 1; protein kinase B; protein tyrosine kinase; 3T3 cell line; adipogenesis; adipose tissue; Article; cell differentiation; disease severity; glucose blood level; human; hyperglycemia; incubation time; proadipocyte; upregulation |
顯示於: | 生化科學研究所 |
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