Hyperglycemia and advanced glycation end products (AGEs) suppress the differentiation of 3T3-L1 preadipocytes
Journal
Oncotarget
Journal Volume
8
Journal Issue
33
Pages
55039 - 55050
Date Issued
2017
Author(s)
Chang, Chia-Chu
Chen, Chen-Yu
Chen, Ting-Huan
Chen, Woan-Ling
Wen, Hui-Chin
Huang, Chih-Yang
Chang, Chung-Ho
Abstract
Aging is characterized by mild hyperglycemia and accumulation of advanced glycation end products (AGEs). Effects of chronic exposure to hyperglycemia or AGEs on the adipogenic differentiation of 3T3-L1 preadipocytes remain unclear. We examined the chronic effect of AGEs and high glucose on the differentiation of 3T3-L1 cells by culturing 3T3-L1 cells in the presence of AGEs or 25 mM glucose for 1 month. Chronic incubation of 3T3-L1 cells with AGEs or high glucose blocked their differentiation into mature adipocytes as evidenced by reduced levels of adipocyte markers such as accumulated oil droplets, GPDH, aP2, adiponectin and of adipogenesis regulators PPARγ and C/EBPα. Levels or activities of Src, PDK1, Akt, and NF-κB were higher in AGEs- and high glucose-treated cells than those in 3T3-L1 cells. Levels of Bcl-2 were elevated in AGEs- and high glucose-treated cells, and were attenuated by inhibitors of PI3-kinase, Akt and NF-κB. Moreover, adipogenesis was attenuated in 3T3-L1 cells stably expressing Bcl-2 or YAP. These results suggest that chronic AGEs and high glucose treatments up-regulate Bcl-2 and YAP via the Akt-NF-κB pathway and impair adipogenesis. ? Chang et al.
Subjects
Adipogenesis; Advanced glycation end products (AGEs); Bcl-2; Hyperglycemia; NF-kB
SDGs
Other Subjects
adiponectin; advanced glycation end product; glucose; immunoglobulin enhancer binding protein; peroxisome proliferator activated receptor gamma; phosphatidylinositol 3 kinase; phosphoinositide dependent protein kinase 1; protein kinase B; protein tyrosine kinase; 3T3 cell line; adipogenesis; adipose tissue; Article; cell differentiation; disease severity; glucose blood level; human; hyperglycemia; incubation time; proadipocyte; upregulation
Type
journal article