https://scholars.lib.ntu.edu.tw/handle/123456789/457019
標題: | Isolation and identification of post-transcriptional gene silencing-related micro-RNAs by functionalized silicon nanowire field-effect transistor | 作者: | Chen K.-I. Pan C.-Y. Li K.-H. Huang Y.-C. Lu C.-W. Tang C.-Y. Su Y.-W. Tseng L.-W. Tseng K.-C. Lin C.-Y. Chen C.-D. Lin S.-S. YIT-TSONG CHEN SHIH-SHUN LIN CHIEN-YUAN PAN |
公開日期: | 2015 | 卷: | 5 | 來源出版物: | Scientific Reports | 摘要: | Many transcribed RNAs are non-coding RNAs, including microRNAs (miRNAs), which bind to complementary sequences on messenger RNAs to regulate the translation efficacy. Therefore, identifying the miRNAs expressed in cells/organisms aids in understanding genetic control in cells/organisms. In this report, we determined the binding of oligonucleotides to a receptor-modified silicon nanowire field-effect transistor (SiNW-FET) by monitoring the changes in conductance of the SiNW-FET. We first modified a SiNW-FET with a DNA probe to directly and selectively detect the complementary miRNA in cell lysates. This SiNW-FET device has 7-fold higher sensitivity than reverse transcription-quantitative polymerase chain reaction in detecting the corresponding miRNA. Next, we anchored viral p19 proteins, which bind the double-strand small RNAs (ds-sRNAs), on the SiNW-FET. By perfusing the device with synthesized ds-sRNAs of different pairing statuses, the dissociation constants revealed that the nucleotides at the 3′-overhangs and pairings at the terminus are important for the interactions. After perfusing the total RNA mixture extracted from Nicotiana benthamiana across the device, this device could enrich the ds-sRNAs for sequence analysis. Finally, this bionanoelectronic SiNW-FET, which is able to isolate and identify the interacting protein-RNA, adds an additional tool in genomic technology for the future study of direct biomolecular interactions. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/457019 | ISSN: | 20452322 | DOI: | 10.1038/srep17375 | SDG/關鍵字: | microRNA; nanowire; silicon; chemistry; conformation; devices; gene expression profiling; gene silencing; genetics; nanotechnology; procedures; RNA interference; RNA processing; transistor; Gene Expression Profiling; Gene Silencing; MicroRNAs; Nanotechnology; Nanowires; Nucleic Acid Conformation; RNA Interference; RNA Processing, Post-Transcriptional; Silicon; Transistors, Electronic |
顯示於: | 生物科技研究所 |
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