https://scholars.lib.ntu.edu.tw/handle/123456789/462204
Title: | Suppression of fructose-bisphosphate aldolase C expression as a predictor of advanced oral squamous cell carcinoma | Authors: | Li Y.-J. Huang T.-H. Hsiao M. BEEN-REN LIN SHIH-JUNG CHENG Yang C.-N. Lai W.-T. Wu T.-S. Fan J.-R. YEN-PING KUO Chang C.-C. |
Issue Date: | 2016 | Publisher: | John Wiley and Sons Inc. | Journal Volume: | 38 | Start page/Pages: | E1075-E1085 | Source: | Head and Neck | Abstract: | Background Glycolysis machinery regulates cancer cell behavior. However, the roles of these glycolysis enzymes in oral squamous cell carcinoma (OSCC) progression remain unknown. Methods Fructose-bisphosphate aldolase C (ALDOC) expression in OSCC patients and cell lines was detected using quantitative real-time polymerase chain reaction (PCR). The functions of ALDOC in migration and invasion were determined using gain and loss of function approaches. An orthotopic OSCC animal model was performed to investigate the effects of ALDOC on metastasis and tumorigenesis in vivo. Results ALDOC expression is negatively significantly correlated with clinical outcome and cell migration in vitro and in vivo. ALDOC blocks adenosine triphosphate generation and lactate production, and mutation constructs of Arg42 and Lys146 functionally restore ALDOC-inhibited cell migration and invasion. Conclusion ALDOC functions as an OSCC prognosis marker clinically, and suppresses migration and invasion by its catalytic domain of Arg42 and Lys146. ? 2015 Wiley Periodicals, Inc.. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84973462488&doi=10.1002%2fhed.24161&partnerID=40&md5=592d5c1209d65d16a1a779ba593e7a6d https://scholars.lib.ntu.edu.tw/handle/123456789/462204 |
ISSN: | 1043-3074 | DOI: | 10.1002/hed.24161 | SDG/Keyword: | adenosine triphosphate; arginine; fructose bisphosphate aldolase; fructose bisphosphate aldolase c; lactic acid; lysine; unclassified drug; fructose bisphosphate aldolase; adult; advanced cancer; animal experiment; animal model; Article; cancer growth; cancer prognosis; cancer staging; cancer survival; carcinogenesis; cell invasion; cell migration; cell motility; controlled study; disease free survival; enzyme repression; female; gene expression; genetic transfection; human; human cell; in vitro study; in vivo study; lymph node metastasis; major clinical study; male; middle aged; mouse; mouth squamous cell carcinoma; nonhuman; overall survival; prediction; priority journal; real time polymerase chain reaction; tumor xenograft; animal; cell motion; enzymology; metabolism; mouth tumor; mutation; nonobese diabetic mouse; prognosis; SCID mouse; squamous cell carcinoma; tumor cell line; tumor invasion; Animals; Carcinoma, Squamous Cell; Cell Line, Tumor; Cell Movement; Female; Fructose-Bisphosphate Aldolase; Humans; Male; Mice, Inbred NOD; Mice, SCID; Middle Aged; Mouth Neoplasms; Mutation; Neoplasm Invasiveness; Prognosis; Real-Time Polymerase Chain Reaction |
Appears in Collections: | 醫學系 |
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