https://scholars.lib.ntu.edu.tw/handle/123456789/463966
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Sadhasivam, S. | en_US |
dc.contributor.author | Chen, J.-C. | en_US |
dc.contributor.author | Savitha, S. | en_US |
dc.contributor.author | Lin, F.-H. | en_US |
dc.contributor.author | Yang, Y.-Y. | en_US |
dc.contributor.author | Lee, C.-H. | en_US |
dc.contributor.author | Lin, Feng-Huei | en_US |
dc.creator | Sadhasivam, S.;Chen, J.-C.;Savitha, S.;Lin, F.-H.;Yang, Y.-Y.;Lee, C.-H.Feng-Huei Lin | - |
dc.date.accessioned | 2020-02-26T01:33:06Z | - |
dc.date.available | 2020-02-26T01:33:06Z | - |
dc.date.issued | 2012 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/463966 | - |
dc.description.abstract | In this study, we developed a sensitive quartz crystal microbalance (QCM) sensor which employs the anti-ovarian tumor associated antigen 1 (OVTA 1) IgY polyclonal chicken antibodies on the crystal surface for the detection of OVTA 1 in human serum. The anti-OVTA 1 IgY antibodies were anchored on the thiol-activated sensor surface using 1-ethyl-3[3-dimethylaminopropyl] carbodiimide hydrochloride/N-hydroxysuccinimide (EDC-NHS) coupling. The responses for different concentrations of anti-OVTA 1 antibody were also studied and 40 μg/mL was favorable for homogenous antibody coverage. The QCM sensor was used to monitor both immobilization and the target binding affinity. The anti-OVTA 1 antibody based QCM has allowed the competent detection of OVTA 1 in a high linear range of 0.5-10 μg/mL. The total time needed to complete the detection was as short as 5-6 h. The regeneration studies demonstrated that the proposed sensor was reusable up to 9 cycles with a slight loss in binding affinity. The detection of OVTA 1 in human serum allows a potential exploitation of the anti-OVTA 1 polyclonal antibody based QCM immunoassay for the screening of OVTA 1 antigen. ? 2012 Elsevier B.V. All rights reserved. | - |
dc.relation.ispartof | Materials Science and Engineering C | - |
dc.subject | Human serum; Immunosensors; Ovarian cancer; OVTA 1 antigen; Polyclonal chicken IgY antibodies; QCM | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | Binding affinities; Carbodiimide hydrochlorides; Crystal surfaces; Human serum; Linear range; Ovarian cancers; Ovarian tumor; Polyclonal; Polyclonal antibody; QCM; QCM sensors; Quartz crystal microbalance sensors; Real-time detection; Sensor surfaces; Target binding; Animals; Antigens; Binding energy; Body fluids; Chemical detection; Diagnosis; Immunosensors; Quartz crystal microbalances; Tumors; Antibodies | - |
dc.title | A real time detection of the ovarian tumor associated antigen 1 (OVTA 1) in human serum by quartz crystal microbalance immobilized with anti-OVTA 1 polyclonal chicken IgY antibodies | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.msec.2012.05.043 | - |
dc.identifier.scopus | 2-s2.0-84863306991 | - |
dc.identifier.url | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84863306991&doi=10.1016%2fj.msec.2012.05.043&partnerID=40&md5=a82aa66345fcf3da8a57765d01a2c9e1 | - |
dc.relation.pages | 2073-2078 | - |
dc.relation.journalvolume | 32 | - |
dc.relation.journalissue | 7 | - |
item.fulltext | no fulltext | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Biomedical Engineering | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Engineering | - |
顯示於: | 醫學工程學研究所 |
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