https://scholars.lib.ntu.edu.tw/handle/123456789/463966
Title: | A real time detection of the ovarian tumor associated antigen 1 (OVTA 1) in human serum by quartz crystal microbalance immobilized with anti-OVTA 1 polyclonal chicken IgY antibodies | Authors: | Sadhasivam, S. Chen, J.-C. Savitha, S. Lin, F.-H. Yang, Y.-Y. Lee, C.-H. Lin, Feng-Huei |
Keywords: | Human serum; Immunosensors; Ovarian cancer; OVTA 1 antigen; Polyclonal chicken IgY antibodies; QCM | Issue Date: | 2012 | Journal Volume: | 32 | Journal Issue: | 7 | Start page/Pages: | 2073-2078 | Source: | Materials Science and Engineering C | Abstract: | In this study, we developed a sensitive quartz crystal microbalance (QCM) sensor which employs the anti-ovarian tumor associated antigen 1 (OVTA 1) IgY polyclonal chicken antibodies on the crystal surface for the detection of OVTA 1 in human serum. The anti-OVTA 1 IgY antibodies were anchored on the thiol-activated sensor surface using 1-ethyl-3[3-dimethylaminopropyl] carbodiimide hydrochloride/N-hydroxysuccinimide (EDC-NHS) coupling. The responses for different concentrations of anti-OVTA 1 antibody were also studied and 40 μg/mL was favorable for homogenous antibody coverage. The QCM sensor was used to monitor both immobilization and the target binding affinity. The anti-OVTA 1 antibody based QCM has allowed the competent detection of OVTA 1 in a high linear range of 0.5-10 μg/mL. The total time needed to complete the detection was as short as 5-6 h. The regeneration studies demonstrated that the proposed sensor was reusable up to 9 cycles with a slight loss in binding affinity. The detection of OVTA 1 in human serum allows a potential exploitation of the anti-OVTA 1 polyclonal antibody based QCM immunoassay for the screening of OVTA 1 antigen. ? 2012 Elsevier B.V. All rights reserved. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/463966 | DOI: | 10.1016/j.msec.2012.05.043 | SDG/Keyword: | Binding affinities; Carbodiimide hydrochlorides; Crystal surfaces; Human serum; Linear range; Ovarian cancers; Ovarian tumor; Polyclonal; Polyclonal antibody; QCM; QCM sensors; Quartz crystal microbalance sensors; Real-time detection; Sensor surfaces; Target binding; Animals; Antigens; Binding energy; Body fluids; Chemical detection; Diagnosis; Immunosensors; Quartz crystal microbalances; Tumors; Antibodies |
Appears in Collections: | 醫學工程學研究所 |
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