https://scholars.lib.ntu.edu.tw/handle/123456789/483284
標題: | Focused shockwave induced blood-brain barrier opening and transfection | 作者: | Kung Y. Lan C. MING-YEN HSIAO Sun M.-K. Hsu Y.-H. ABEL PO-HAO HUANG Liao W.-H. Liu H.-L. Inserra C. WEN-SHIANG CHEN HAO-LI LIU Y Kung C Lan MK Sun YH Hsu APH Liao WH Liao C Inserra WS Chen |
公開日期: | 2018 | 出版社: | Nature Publishing Group | 卷: | 8 | 期: | 1 | 起(迄)頁: | 2218 | 來源出版物: | Scientific Reports | 摘要: | Despite extensive efforts in recent years, the blood-brain barrier (BBB) remains a significant obstacle for drug delivery. This study proposes using a clinical extracorporeal shockwave instrument to open the BBB, combined with a laser assisted bi-axial locating platform to achieve non-invasive, controllable-focus and reversible BBB opening in the brains of rats. Under shockwave treatment with an intensity level of 5 (P-9.79 MPa, energy flux density (EFD) 0.21 mJ/mm2) and a pulse repetition frequency of 5 Hz, the BBB could be opened after 50 shocks without the use of an ultrasound contrast agent. With the proposed method, the BBB opening can be precisely controlled in terms of depth, size and location. Moreover, a shockwave based gene transfection was demonstrated using a luciferase gene. © 2018 The Author(s). |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041558908&doi=10.1038%2fs41598-018-20672-y&partnerID=40&md5=b1882cfc73afd554fdabee64c766d835 https://scholars.lib.ntu.edu.tw/handle/123456789/483284 |
ISSN: | 2045-2322 | DOI: | 10.1038/s41598-018-20672-y | SDG/關鍵字: | luciferase; animal; blood brain barrier; brain; gene therapy; genetic transfection; genetics; high-energy shock wave; procedures; radiation response; rat; reporter gene; shock wave therapy; Animals; Blood-Brain Barrier; Brain; Extracorporeal Shockwave Therapy; Genes, Reporter; Genetic Therapy; High-Energy Shock Waves; Luciferases; Rats; Transfection |
顯示於: | 醫學系 |
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