Focused shockwave induced blood-brain barrier opening and transfection
Journal
Scientific Reports
Journal Volume
8
Journal Issue
1
Pages
2218
Date Issued
2018
Author(s)
Kung Y.
Lan C.
Sun M.-K.
Hsu Y.-H.
Liao W.-H.
Liu H.-L.
Inserra C.
Y Kung
C Lan
MK Sun
YH Hsu
APH Liao
WH Liao
C Inserra
WS Chen
Abstract
Despite extensive efforts in recent years, the blood-brain barrier (BBB) remains a significant obstacle for drug delivery. This study proposes using a clinical extracorporeal shockwave instrument to open the BBB, combined with a laser assisted bi-axial locating platform to achieve non-invasive, controllable-focus and reversible BBB opening in the brains of rats. Under shockwave treatment with an intensity level of 5 (P-9.79 MPa, energy flux density (EFD) 0.21 mJ/mm2) and a pulse repetition frequency of 5 Hz, the BBB could be opened after 50 shocks without the use of an ultrasound contrast agent. With the proposed method, the BBB opening can be precisely controlled in terms of depth, size and location. Moreover, a shockwave based gene transfection was demonstrated using a luciferase gene. © 2018 The Author(s).
SDGs
Other Subjects
luciferase; animal; blood brain barrier; brain; gene therapy; genetic transfection; genetics; high-energy shock wave; procedures; radiation response; rat; reporter gene; shock wave therapy; Animals; Blood-Brain Barrier; Brain; Extracorporeal Shockwave Therapy; Genes, Reporter; Genetic Therapy; High-Energy Shock Waves; Luciferases; Rats; Transfection
Publisher
Nature Publishing Group
Type
journal article